MgADP binding to the high-affinity 'consensus' ATPase active site of SUR1 and remodeling of the L0-loop (lasso region) overrides tonic ATP inhibition of KATP channels.
Ben C Tilly, Gillian E Chalkley ... C Peter Verrijzer
Visualization of BRM remodelers engaging their endogenous genomic targets revealed highly transient and dynamic interactions with chromatin, which are fueled by ATP-hydrolysis.
Michael H Hayes, Elizabeth H Peuchen ... Daniel L Weeks
The equilibrium between solubility and aggregation of proteins in the nucleus is controlled by co-aggregates like RNA and the action of ATP as both an energy source and a destabilizing chemical agent.
Biochemical analysis on hetero-mutated c10 subunits ring and molecular dynamics simulations demonstrate the cooperation among c-subunits of FoF1-ATP synthase in rotation-coupled proton translocation.
Maria A Prusicki, Emma M Keizer ... Arp Schnittger
Live cell imaging and the in vivo dissection into distinct stages, called landmarks, represents a new methodology for analyzing meiosis in Arabidopsis and likely in other species as well.
Rachael M Barry, Anne-Florence Bitbol ... Zemer Gitai
The accumulation of its product, cytidine triphosphate, encourages the enzyme CTP synthetase (CtpS) to form large-scale polymers and inhibits the enzyme's activity.
Meghna Sobti, Robert Ishmukhametov ... Alastair G Stewart
Cryo-EM studies reveal that incubation with ATP produces conformational intermediates of E. coli ATP synthase, in which the ε subunit is no longer in its autoinhibitory conformation.
Structural analysis of the ATP synthase – in combination with evolutionary covariance analysis – reveals the fold of the a subunit and shows that the enzyme can adopt several different conformations, which support the Brownian ratchet model for generating rotation.
Joseph Atherton, Irene Farabella ... Carolyn A Moores
Cryo-electron microscopy reconstructions of two microtubule-bound transport kinesins at 7 Å resolution reveal how microtubule track binding stimulates ADP release, primes the active site for ATP binding and enables force generation.