During CRISPR adaptation, Cas4 forms a ternary complex with the Cas1-Cas2 spacer integration complex, an interaction that coordinates substrate hand-off following precise, PAM-dependent prespacer processing prior to integration.
Lindsay B Case, Milagros De Pasquale ... Michael K Rosen
Biochemical and cell biological data suggest a model of nascent integrin adhesion complex formation based on synergistic phase separation of pathways surrounding the focal adhesion kinase (FAK) and the adaptor protein p130Cas.
Kevin J Forsberg, Ishan V Bhatt ... Harmit S Malik
A high-throughput, activity-based selection identifies many phage-derived inhibitors of Cas9 from human fecal and oral metagenomes, with at least one inhibitor acting via a novel mechanism.
The tetrameric structure of a casposase bound to DNA and its biochemical properties show how a transposase could have evolved to perform CRISPR-Cas spacer acquisition.
Hironori Uehara, Xiaohui Zhang ... Balamurali K Ambati
Adenoviral CRISPR/Cas9 gene knockdown of mutant Col8a2 achieves structural and functional rescue of murine Fuchs’ endothelial corneal dystrophy, the first demonstration of post-mitotic CRISPR-based knockdown for phenotypic restoration.
Two regions of the frontal lobe – the anterior cingulate cortex and, unexpectedly, the dorsolateral prefrontal cortex – show coordinated rhythmic activity during REM sleep.