13,316 results found
    1. Chromosomes and Gene Expression

    Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair

    Natasa Savic, Femke CAS Ringnalda ... Gerald Schwank
    Linking the DNA repair template to the Cas9 ribonucleoprotein complex enhances homology-directed repair of the induced DNA double strand break.
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    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    The Cas4-Cas1-Cas2 complex mediates precise prespacer processing during CRISPR adaptation

    Hayun Lee, Yukti Dhingra, Dipali G Sashital
    During CRISPR adaptation, Cas4 forms a ternary complex with the Cas1-Cas2 spacer integration complex, an interaction that coordinates substrate hand-off following precise, PAM-dependent prespacer processing prior to integration.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation

    Lindsay B Case, Milagros De Pasquale ... Michael K Rosen
    Biochemical and cell biological data suggest a model of nascent integrin adhesion complex formation based on synergistic phase separation of pathways surrounding the focal adhesion kinase (FAK) and the adaptor protein p130Cas.
    1. Microbiology and Infectious Disease

    Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome

    Kevin J Forsberg, Ishan V Bhatt ... Harmit S Malik
    A high-throughput, activity-based selection identifies many phage-derived inhibitors of Cas9 from human fecal and oral metagenomes, with at least one inhibitor acting via a novel mechanism.
    1. Structural Biology and Molecular Biophysics

    Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas

    Alison B Hickman, Shweta Kailasan ... Fred Dyda
    The tetrameric structure of a casposase bound to DNA and its biochemical properties show how a transposase could have evolved to perform CRISPR-Cas spacer acquisition.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Molecular mechanism of active Cas7-11 in processing CRISPR RNA and interfering target RNA

    Hemant N Goswami, Jay Rai ... Hong Li
    Three-dimensional structure of CRISPR Cas7-11 shed light on protein evolution and guide development of biotechnology for RNA manipulations in cells.
    1. Genetics and Genomics

    Start codon disruption with CRISPR/Cas9 prevents murine Fuchs’ endothelial corneal dystrophy

    Hironori Uehara, Xiaohui Zhang ... Balamurali K Ambati
    Adenoviral CRISPR/Cas9 gene knockdown of mutant Col8a2 achieves structural and functional rescue of murine Fuchs’ endothelial corneal dystrophy, the first demonstration of post-mitotic CRISPR-based knockdown for phenotypic restoration.
    1. Microbiology and Infectious Disease

    Type III CRISPR-Cas systems can provide redundancy to counteract viral escape from type I systems

    Sukrit Silas, Patricia Lucas-Elio ... Antonio Sánchez-Amat
    Cooperation between evolutionarily disparate CRISPR-Cas modules allows bacteria to counter mutational escape by viruses.
    1. Genetics and Genomics

    Closely related type II-C Cas9 orthologs recognize diverse PAMs

    Jingjing Wei, Linghui Hou ... Yongming Wang
    Closely related type II-C Cas9 orthologs recognize diverse PAMs, which facilitates us to expand the targeting scope.
    1. Neuroscience

    Frontal beta-theta network during REM sleep

    Sujith Vijayan, Kyle Q Lepage ... Sydney S Cash
    Two regions of the frontal lobe – the anterior cingulate cortex and, unexpectedly, the dorsolateral prefrontal cortex – show coordinated rhythmic activity during REM sleep.

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