During centrosome maturation, pericentrin is delivered to the centrosome co-translationally by a microtubule- and dynein-dependent process, as pericentrin mRNA is undergoing active translation near the centrosome.
Ines Alvarez-Rodrigo, Thomas L Steinacker ... Jordan W Raff
Polo kinase recruitment by the protein Spd-2 to the pericentriolar scaffold is a key element of the positive feedback loop which drives the expansion of the mitotic centrosomes in flies.
Paul T Conduit, Jennifer H Richens ... Jordan W Raff
The proteins DSpd-2 and Centrosomin assemble into dynamic scaffolds that build from the inside out around the mother centriole and support centrosome maturation.
The microtubule organizing potential of the centrosome is inactivated in a stepwise process through phosphatase activity and mechanical disruption to remove an aging matrix of proteins.
Newly discovered interaction between fission yeast SPB and animal centriole components reveals that pericentrin not only functions as a microtubule-nucleator, but also promotes centriole assembly in animals.
The polarized orientation of the mitotic spindle in budding yeast arises from spindle pole structural and functional asymmetry subject to cell cycle control.