Label-free three-photon imaging of whole, uncleared intact organoids generated from Rett syndrome patients show shorter migration distances and slower migration speeds of mutant radially migrating neurons with more tortuous trajectories.

Combining cerebral organoid technology with cryo-correlative microscopy reveals the organization of cytoskeleton, membrane compartments, and protein synthesis machinery contributing to the rapid expansion of developing human axons.

Increased excitation and decreased inhibition associated with abnormal neuronal morphology, aberrant ion channel properties, and synaptic dysfunction contribute to hyperexcitability in Alzheimer’s disease hiPSC-derived neuronal cultures and cerebral organoids.

Neural progenitors in humans and chimpanzee organoids show remarkably similar cellular and molecular parameters, but metaphase is longer during human mitosis.

Chimeric RNAs are widely distributed spatiotemporally during human retinal development and have important regulatory functions, such as silencing of CTNNBIP1-CLSTN1 biasing the progenitor cells toward the RPE cell fate at the expense of neural retinal cell fates.

A bioengineering approach identifies tissue morphology as an effective variable for controlling the inception of neural organoid morphogenesis via induction of a biomimetic, singular neural rosette tissue cytoarchitecture.

RNA-protein multiome approach helps to discover that the posttranscriptional regulation of the translational machinery is crucial for the fidelity of cortical development.

Iodixanol supplementation provides a simple method for tuning the refractive index of live imaging media, which can greatly improve resolution and penetration depth in live imaging experiments.

Automated liquid handling, whole mount staining, and clearing allow unbiased 3D quantitation of cell markers in human neural organoids with diameters of up to 1 mm at the single-cell level.

A vessel and brain fusion organoid model provides a platform for the study of interactions between neuronal and non-neuronal components during brain development and functioning.