Veronica Teresa Ober, George Boniface Githure ... Michael Boshart
A minimal subset of two to three residues in cyclic nucleotide binding (CNB) domains controls nucleoside vs. cyclic nucleotide specificity, repurposing PKA of certain pathogens for novel nucleoside signaling pathways or sensing.
Zachary F Hallberg, Chi Ho Chan ... Ming C Hammond
A newfound signaling enzyme that diverged from a protein family ubiquitous in bacteria provides mechanistic insights into how new signaling activity emerges to control distinct cellular function and physiology.
The crystal structure of a mammalian protein kinase G reveals contacts between the regulatory and catalytic domains, indicates how cGMP binding alters domain conformations and thus domain:domain interactions, and informs a model for enzyme auto-inhibition and cooperative activation.
Marcel P Goldschen-Ohm, Vadim A Klenchin ... Baron Chanda
Single-molecule resolution of cAMP binding to the ligand binding domain of pacemaking channels in zero-mode waveguides reveals the dynamics of the distinct steps underlying both binding and isomerization of the binding domain.
Melanopsin phototransduction targets distinct complements of transduction channels across intrinsically photosensitive retinal ganglion cell subtypes and does not require hyperpolarization-activated cyclic nucleotide-gated channels.
Alice L Herneisen, Zhu-Hong Li ... Sebastian Lourido
Time-resolved phosphoproteomics and thermal proteome profiling reveal the Ca2+-responsive proteome of the model apicomplexan Taxoplasma gondii, identifying PP1 as a Ca2+-responsive enzyme that regulates Ca2+ uptake to promote parasite motility.