202 results found
    1. Biochemistry and Chemical Biology

    Structural and functional characterization of G protein–coupled receptors with deep mutational scanning

    Eric M Jones, Nathan B Lubock ... Sriram Kosuri
    Linking deep mutational scanning with engineered transcriptional reporters in human cell lines establishes a generalizable method for exploring pharmacogenomics, structure, and function across broad classes of drug receptors.
    1. Evolutionary Biology
    2. Microbiology and Infectious Disease

    The inherent mutational tolerance and antigenic evolvability of influenza hemagglutinin

    Bargavi Thyagarajan, Jesse D Bloom
    Deep mutational scanning was used to comprehensively quantify the effects of mutations to influenza hemagglutinin and shows that the virus possesses a high inherent mutational tolerance at key antigenic sites.
    1. Genetics and Genomics
    2. Structural Biology and Molecular Biophysics

    Determinants of trafficking, conduction, and disease within a K+ channel revealed through multiparametric deep mutational scanning

    Willow Coyote-Maestas, David Nedrud ... Daniel Schmidt
    By measuring the impacts of thousands of mutations on potassium channel trafficking and function, we illuminate the molecular basis of folding, structure–function relationships, and how these are altered in disease.
    1. Evolutionary Biology
    2. Microbiology and Infectious Disease

    Mapping mutational effects along the evolutionary landscape of HIV envelope

    Hugh K Haddox, Adam S Dingens ... Jesse D Bloom
    Deep mutational scanning of Env proteins from two transmitted-founder strains of HIV shows how the accessible evolutionary space changes as the virus evolves.
    1. Biochemistry and Chemical Biology

    Mapping the functional landscape of the receptor binding domain of T7 bacteriophage by deep mutational scanning

    Phil Huss, Anthony Meger ... Srivatsan Raman
    An unbiased mutational screen decodes the molecular basis of phage receptor interactions, identifies key functional regions determining activity and host range, and demonstrates the potential for engineering therapeutic phages.
    1. Computational and Systems Biology
    2. Structural Biology and Molecular Biophysics

    Deep mutational scanning and machine learning reveal structural and molecular rules governing allosteric hotspots in homologous proteins

    Megan Leander, Zhuang Liu ... Srivatsan Raman
    Deep mutational scanning of homologous proteins shows conservation in allosteric mechanisms but differences in molecular details within the protein family.
    1. Biochemistry and Chemical Biology
    2. Computational and Systems Biology

    Comprehensive exploration of the translocation, stability and substrate recognition requirements in VIM-2 lactamase

    John Z Chen, Douglas M Fowler, Nobuhiko Tokuriki
    Comprehensive mutational characterisation of VIM-2 metallo-beta-lactamase deciphered the residues responsible for key functional behavior, including translocation, stability, substrate recognition and catalysis.
    1. Biochemistry and Chemical Biology
    2. Evolutionary Biology

    Enhanced ER proteostasis and temperature differentially impact the mutational tolerance of influenza hemagglutinin

    Angela M Phillips, Michael B Doud ... Matthew D Shoulders
    Endoplasmic reticulum proteostasis factors enhance the mutational tolerance of influenza hemagglutinin, a model secretory pathway protein and therapeutic target, particularly improving the fitness of temperature-sensitive variants.
    1. Evolutionary Biology
    2. Microbiology and Infectious Disease

    Antigenic evolution of human influenza H3N2 neuraminidase is constrained by charge balancing

    Yiquan Wang, Ruipeng Lei ... Nicholas C Wu
    The local fitness landscape of an antigenic region in influenza neuraminidase was determined by combinatorial mutagenesis and next-generation sequencing.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context

    Atanas Radkov, Anne L Sapiro ... Seemay Chou
    Distinct surfaces of an interbacterial competition cell wall toxin mediate interactions with different cellular binding partners, resulting in an inherent evolutionary trade-off across the toxin superfamily.

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