Claudia C Carcamo, Matthew F Poyton ... Taekjip Ha
In vitro single-particle tracking reveals that ATP binding increases the one-dimensional diffusion of yeast chromatin remodeler SWR1 on DNA stretched between optical tweezers and diffusion is confined by protein roadblocks and nucleosomes.
Compared to other isoforms of RAS, KRAS4b has unique membrane diffusion behavior related to its biology, and the two major domains of the protein both contribute to this diffusion behavior.
Moritz Armbruster, Chris G Dulla, Jeffrey S Diamond
Fluorescent glutamate indicators distort the time course of neurotransmitter diffusion and uptake by competing with transporters, an important caveat to consider when using iGluSnFR and its analogs.
Realistic reaction-diffusion signaling networks that include cell-autonomous factors can robustly form self-organizing spatial patterns for any combination of diffusion coefficients without requiring differential diffusivity.
Cell-specific architectural properties such as the axon diameter of the white matter of the human brain can be quantified accurately and non-invasively using diffusion magnetic resonance imaging.
The evolving spatial distribution of nuclei between apical and basal surfaces of the developing retinal neuroepithelium is quantitatively described by a nonlinear diffusion equation accounting for crowding within the tissue.
Nicola Bellotto, Jaime Agudo-Canalejo ... Victor Sourjik
Diffusion of differently sized proteins in bacterial cytoplasm is nearly Brownian and consistent with the Stokes-Einstein relation, once protein shape and cell geometry are taken into account, and effects of various perturbation can be described as changes in cytoplasmic viscosity.
The collective dynamics of cell signaling relays are at once dramatically sensitive to the system dimensionality and insensitive to many biological details.
Tracking proteins in live cells is challenging due to technical limitations and biological complexity, but approaches based in Bayesian nonparametrics stand a decent chance at recovering a target protein's dynamic profile from noisy data.
