Rigorous biochemical and structural analyses reveal the precise topology of cohesin's association with DNA and suggest a mechanism for how DNA is transported inside the ring.
Linda V Sinclair, Andrew JM Howden ... Doreen A Cantrell
Antigen receptor control of methionine transport is critical to co-ordinate protein synthesis and the production of methyl donors for nucleotide and protein methylations which are required for T cell differentiation.
Conservative mutations within a secondary active transport protein influenced the conformational equilibrium probed using NMR spectroscopy which correlated to the functional output in vivo.
Studies of the substrate selectivity of concentrative nucleoside transporters provide proof of principle for structure-based improvement of drug delivery by these transporters.
An optimized CRISPR-Cas9 system enables multiplexed genome engineering for evolving biomolecules and pathways from chromosomally integrated DNA libraries.
Aaron T Bozzi, Christina M Zimanyi ... Rachelle Gaudet
Nramp-family transporters adapt a common fold to a novel mechanism in which the spatial and temporal separation of like-charge transition metal and proton co-substrates circumvents the expected electrostatic repulsion.
Joana A Santos, Stephan Rempel ... Dirk J Slotboom
ECF-CbrT is a bacterial vitamin B12 transporter that is structurally different from the well-characterized transporter BtuCDF, yet has similar functional properties.
Combined cutting-edge technologies discovered a pair of carboxylate transporters that appears evolutionarily different among novel transporters and that is essential for parasite physiology.