Transposition reactions that occur during DNA replication and involve the termini of adjacent transposons can induce genome expansion by re-replication of transposon-flanking sequences.
Biochemical data demonstrate that the conformation of the target site DNA can dramatically modulate the kinetics and directionality of the otherwise isoenergetic transposition reaction of DDE transposases.
The tetrameric structure of a casposase bound to DNA and its biochemical properties show how a transposase could have evolved to perform CRISPR-Cas spacer acquisition.
Genetic, structural, and biochemical analyses of IS607-family transposons shows that the DNA translocation reaction proceeds very differently from other reactions promoted by serine recombinases.
Disintegration, regarded as an abortive side reaction antithetical to DNA transposition, can promote protospacer integration at the CRISPR locus via DNA repair pathways.
Elizabeth R Morris, Heather Grey ... Julia M Richardson
Structural and biochemical analyses of a eukaryotic DNA transpososome in the integration step reveal how mariner/Tc1 transposons are selectively integrated into a TA target sequence.
Tn5 transposase has direct tagmentation activity towards RNA/DNA hybrids, which is harnessed as a more convenient and faster RNA-seq library construction method and will benefit RNA and chromatin research.
Corentin Claeys Bouuaert, Karen Lipkow ... Ronald Chalmers
A DNA transposon, or ‘jumping gene’, controls its amplification within a genome through a competition between the enzyme multimers that are responsible for its mobility.
Satyam P Srivastav, Reazur Rahman ... Nelson C Lau
A female fertility syndrome in Drosophila called gonadal dysgenesis is caused by the P-transposase actively mobilizing a very short P-element variant that has been named the Har-P.
