The tripartite drug efflux pump AcrA-AcrB-TolC, representative of a wide group of pumps from Gram-negative bacteria, enters a transport-competent state through long-distance conformational changes that switch the channel from a closed to an open state.
A transport mechanism is uncovered in the major drug-efflux system in E. coli involving two remote alternating-access conformational cycles, which could provide the basis for the development of allosteric inhibitors against multidrug resistance.
Phenotypic variants that avoid antibiotic accumulation within clonal bacterial populations are a significant contributor to antibiotic treatment failure and can rely on previously unrecognised survival strategies such as fast growth during drug treatment.
Integrated multi-scale, multi-tool approach to design novel 'evolution drugs' which can delay the emergence of antibiotic resistance by constraining bacterial evolutionary escape strategies.
Gabrielle E Giese, Melissa D Walker ... Albertha JM Walhout
Metabolic activity of the methionine/S-adenosylmethionine cycle is sensed and transcriptionally regulated by a nuclear hormone receptor in Caenorhabditis elegans in order to maintain metabolic homeostasis in a tightly controlled regime.
Karla Marie Márquez-Nogueras, Miryam Andrea Hortua Triana ... Silvia NJ Moreno
A transient receptor channel in Toxoplasma gondii that conducts calcium represents the first molecular element that initiates upstream calcium signals that activate parasite pathogenic pathways.
Abhishek Jamwal, Cristina F Constantin ... Matthew K Higgins
The most effective growth-neutralising antibodies which target the PfRH5 protein from a malaria parasite function by preventing the parasite from binding to basigin-containing membrane protein complexes on human erythrocytes.