Ines Hernandez-Perez, Javier Rubio ... María Isabel Geli
Kazrin, a protein widely expressed in vertebrates whose depletion causes defects in cell adhesion and migration, is recruited to early endosomes and promotes dynein/dynactin-dependent traffic of endocytosed cargo to the recycling endosomes.
Manuel Alonso Y Adell, Simona M Migliano ... David Teis
Quantitative 3D lattice light sheet microscopy of unperturbed cells combined with electron tomography and acute loss of function experiments reveals how dynamic ESCRT-III/Vps4 assemblies succeed in reverse membrane budding on endosomes.
CapZ controls actin filament density around immature early endosomes via its C-terminal domain to facilitate the homotypic fusion of the endocytic vesicles, and it functions as a scaffold protein via its N-terminal domain to recruit RAB5 effectors to early endosomes.
Maria Podinovskaia, Cristina Prescianotto-Baschong ... Anne Spang
A novel, inexpensive, easy-to-use method to analyse traffic along the endosomal pathway in mammalian cells shows that there is coordination between Rab conversion and acidification, but almost none between Rab conversion and recycling.
Cells package active receptors in endosomes at fairly constant amounts and can determine different cell-fate decisions by regulating the number and lifetime of receptor packages.
Plant-unique RAB5 effector 2 (PUF2) is an effector of plant-unique ARA6, which plays a key role in plant endosomal transport, integrating functions of the two plant RAB5 groups by an unprecedented mechanism.
Shawn P Shortill, Mia S Frier ... Elizabeth Conibear
The yeast VARP homolog forms a new sorting nexin complex that promotes its own membrane recruitment through GEF activity and regulates the distribution of endosomal proteins.