Anna Leabourn Boss, Tanvi Damani ... Anna ES Brooks
Full spectrum flow cytometry reveals a high degree of placental mesenchymal cell heterogeneity, which is lost with culture, highlighting the importance of detailed ex vivo phenotyping to optimise the use of these cells in downstream applications.
Under fluid shear, living cells deform elliptically, align in flow direction, and rotate, from which their frequency-dependent viscoelastic properties can be inferred.
James W Opzoomer, Jessica A Timms ... Shahram Kordasti
ImmunoCluster permits nonspecialist users to interrogate liquid and imaging mass, and flow cytometry datasets, resolving novel layers of cellular heterogeneity and insight, as well as producing publishable outputs and figures.
A novel binning approach provides evidence for super-functional T cells with high activation potential and which exhibit Tfh-like functionalities and can help B cells.
Andrew W DeVilbiss, Zhiyu Zhao ... Sean J Morrison
A method was developed for the metabolomic analysis of small numbers of flow cytometrically isolated cells from rare cell populations such as hematopoietic stem cells and circulating cancer cells.
Laura Ferrer-Font, Palak Mehta ... Johannes U Mayer
A systematic optimization of intestinal digestion protocols allows for the isolation and high-dimensional flow cytometric analysis of intestinal immune cells during murine helminth infection.