Scalar irradiance microsensor measurements performed inside the tissue of living corals show that absorption and fluorescence emission by host pigments produce dramatic spectral alterations in the light environment experienced by the symbionts.

Widefield fluorescence from the brain arises from greater depth and area than typically appreciated and is usually a weighted average across cortical columns and often more than one cortical area.

A bright and stochastic multicolor labeling method, Tetbow, facilitates millimeters-scale reconstructions of neuronal circuits at a large scale using tissue clearing.

Integrating a cryogenic light microscope with a focused ion beam/scanning electron microscope, allows directly targeting of fluorescent structure when preparing a frozen-hydrated lamella, without the need for repositioning or fiducial markers.

A physics-based, statistically rigorous mathematical model enables automated, objective interpretation of images from single-molecule fluorescence colocalization microscopy experiments.

The mechanistic basis of molecular movement and interactions within plant root cells can be quantified using scanning fluorescence correlation spectroscopy.

Fluorescence lifetime imaging microscopy, paired with fluorescent, voltage-sensitive dyes, provides a method for measuring and quantifying membrane potentials of living cells.

Improved two-photon fluorescence microendoscopy enables volumetric imaging of synapses and neurons in deep brain structures in vivo.

Peptide and nitrate transporters have been converted into fluorescent reporters of transport activity and have been used to measure various transport properties including the dual affinity of the nitrate transceptor

A modified form of Green Fluorescent Protein integrated into an ammonium transporter provides a sensor that can be used to monitor transport activity in vivo.