1,017 results found
    1. Chromosomes and Gene Expression

    Super-resolution imaging of a 2.5 kb non-repetitive DNA in situ in the nuclear genome using molecular beacon probes

    Yanxiang Ni, Bo Cao ... Hanben Niu
    Molecular beacon based FISH shows the capability of visualizing a 2.5 kb non-repetitive genomic DNA sequence in situ in human or mouse nuclear genome at super resolution.
    1. Structural Biology and Molecular Biophysics
    2. Chromosomes and Gene Expression

    Single-molecule tracking of the transcription cycle by sub-second RNA detection

    Zhengjian Zhang, Andrey Revyakin ... Robert Tjian
    A new technique called fastFISH enables nearly real-time and stoichiometric detection of nascent RNA and the tracking of individual stages of transcription at the level of single-molecules.
    1. Neuroscience

    Functional limb muscle innervation prior to cholinergic transmitter specification during early metamorphosis in Xenopus

    Francois M Lambert, Laura Cardoit ... Didier Le Ray
    Molecular labeling, electrophysiology and calcium imaging have revealed a novel switching of neurotransmitter at the frog neuromuscular junction where motoneurons transiently release glutamate before acetylcholine at synapses on developing hindlimb muscles at the onset of metamorphosis.
    1. Immunology and Inflammation

    Somatic hypermutation of T cell receptor α chain contributes to selection in nurse shark thymus

    Jeannine A Ott, Caitlin D Castro ... Michael F Criscitiello
    Shark's T cells suggest plasticity in diversification mechanisms used by vertebrate lymphocytes, including somatic hypermutation in the thymus.
    1. Chromosomes and Gene Expression

    A multiplexed DNA FISH strategy for assessing genome architecture in Caenorhabditis elegans

    Brandon D Fields, Son C Nguyen ... Scott Kennedy
    The design and validation of an oligo-based DNA FISH library that targets the entire Caenorhabditis elegans genome at chromosome, three megabase, and 500 kb scales.
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Absolute quantitation of individual SARS-CoV-2 RNA molecules provides a new paradigm for infection dynamics and variant differences

    Jeffrey Y Lee, Peter AC Wing ... Ilan Davis
    Single-molecule analysis of SARS-CoV-2 RNA identifies significant heterogeneity in cellular viral RNA levels and highlights slower replication kinetics for the Alpha variant compared to the Victoria strain.
    1. Developmental Biology
    2. Neuroscience

    Differentiation signals from glia are fine-tuned to set neuronal numbers during development

    Anadika R Prasad, Inês Lago-Baldaia ... Vilaiwan M Fernandes
    Extrinsic signals establish an invariant and stereotyped pattern of neuronal differentiation and programmed cell death in the Drosophila visual system.
    1. Cell Biology
    2. Developmental Biology

    N-myc downstream regulated gene 1 (ndrg1) functions as a molecular switch for cellular adaptation to hypoxia

    Jong S Park, Austin M Gabel ... Rachel Brewster
    Lactate-Ndrg1a signaling protects the kidney from hypoxic injury and functions partly by inducing reversible degradation of the ATP-demanding sodium-potassium ATPase pump.
    1. Developmental Biology

    Pregnancy success in mice requires appropriate cannabinoid receptor signaling for primary decidua formation

    Yingju Li, Amanda Dewar ... Xiaofei Sun
    Cannabinoid signaling is critical for integration of vascular system and uterine decidualization.

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