The first-in-class cell-permeant fluorescent probe provides direct and visual evidence of SARM1 activation in axonal degeneration and identified the first covalent, allosteric inhibitor of SARM1 acting on the NAD-binding pocket in the ARM domain.

FAST^M is a novel technique that leverages phase-sensitive signal detection for time-resolved multiplexing of probes with millisecond time resolution, opening new opportunities for interrogating rapid cellular signaling and (bio)chemical reactions.

Small probes show that most AMPA receptors are constrained near or in the synapses.

A modified form of Green Fluorescent Protein integrated into an ammonium transporter provides a sensor that can be used to monitor transport activity in vivo.

Protein engineering and large-scale screening has led to the development of sensitive red fluorescent indicators that detect neural activity with high sensitivity in various animal models.

Small molecule antivirals that drive assembly of HBV capsid protein can also bind to pre-assembled capsids causing them to change morphology or even break, suggesting a complex transduction of binding effects across the capsid.

A facile method is implemented to modify membrane proteins with biophysical reporters using cysteine-independent approaches in live cells.

Photobleaching and recovery of a fluorescent glutamate sensor enable demonstration of the distinct spatial architectures of spontaneous versus evoked forms of glutamatergic neurotransmission within single synapses.

The discovery of a fluorescent protein that can be rapidly switched between long-lived ‘on’ and ‘off’ states will lead to a new generation of super-resolution imaging experiments on living cells.