A novel panel of homoFRET biosensors that detect kinase and second messenger activity, called FLAREs, enables multiparameter imaging of signaling activities within a single live cell with a quantitative and ratiometric readout.
Development of a real-time SnRK2 kinase FRET reporter reveals rapid SnRK2 activation by ABA, but not by Methyl-Jasmonate or elevated CO2, while directly demonstrating basal SnRK2 activity in guard cells.
Thomas-O Peulen, Carola S Hengstenberg ... Christian Herrmann
Multimodal spectroscopy (smFRET, EPR, SAXS, and SANS) and integrative structural modeling reveal large-scale domain rearrangements in human guanylate binding protein 1 (hGBP1) that are driving forces for the formation of oligomers that enable its biological function in innate immune defense.
VRAC activation, observed with a FRET sensor of intracellular LRRC8-domains movement during gating and by fluorometry, requires plasma membrane localization and diacylglycerol signaling, but is independent of intracellular ionic strength.
Andrew S LaCroix, Andrew D Lynch ... Brenton D Hoffman
A quantitative understanding of molecular tension sensor function enables the production of unique sensors with desired mechanical properties as well as the ability to distinguish between protein force and protein deformation in mechanosensitive processes.
Shatanik Mukherjee, Vera Jansen ... Dagmar Wachten
A new FRET-based cAMP biosensor with nanomolar sensitivity makes it possible to measure cAMP dynamics in miniscule subcellular compartments like cilia and flagella.