Shatanik Mukherjee, Vera Jansen ... Dagmar Wachten
A new FRET-based cAMP biosensor with nanomolar sensitivity makes it possible to measure cAMP dynamics in miniscule subcellular compartments like cilia and flagella.
A novel panel of homoFRET biosensors that detect kinase and second messenger activity, called FLAREs, enables multiparameter imaging of signaling activities within a single live cell with a quantitative and ratiometric readout.
Unique biosensor design and protein-engineering enables direct visualization of the active form of Fyn kinase with high specificity, minimal perturbation and shows cellular signaling to be compartmentalized and pulsatile.
Jeremiah Keyes, Ambhighainath Ganesan ... Jin Zhang
An individual extracellular signal regulates multiple cellular actions through differences in the temporal dynamics of spatially distinct populations of the central signaling enzyme, extracellular-signal regulated kinase.
Development of nanoluciferase complementation-based tau biosensors to detect tau conformational change and oligomerization in living cells in high-throughput assays.
Trafficking of optogenetic tools to primary cilia using nanobodies allows to study cAMP signaling with spatial and temporal resolution independent of the cell body.
Image-based motion correction enables the use of fluorescence lifetime and other functional imaging modalities in an intravital and clinical context in the presence of physiological motion.
Stephanie E Crilly, Wooree Ko ... Manojkumar A Puthenveedu
A GPCR activated by the same agonist engages different conformational biosensors at different subcellular locations, suggesting that location-based conformations drive spatial encoding of signaling.