107 results found
    1. Cell Biology

    A novel biosensor to study cAMP dynamics in cilia and flagella

    Shatanik Mukherjee, Vera Jansen ... Dagmar Wachten
    A new FRET-based cAMP biosensor with nanomolar sensitivity makes it possible to measure cAMP dynamics in miniscule subcellular compartments like cilia and flagella.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Single-color, ratiometric biosensors for detecting signaling activities in live cells

    Brian L Ross, Brian Tenner ... Jin Zhang
    A novel panel of homoFRET biosensors that detect kinase and second messenger activity, called FLAREs, enables multiparameter imaging of signaling activities within a single live cell with a quantitative and ratiometric readout.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    A Fyn biosensor reveals pulsatile, spatially localized kinase activity and signaling crosstalk in live mammalian cells

    Ananya Mukherjee, Randhir Singh ... Akash Gulyani
    Unique biosensor design and protein-engineering enables direct visualization of the active form of Fyn kinase with high specificity, minimal perturbation and shows cellular signaling to be compartmentalized and pulsatile.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Signaling diversity enabled by Rap1-regulated plasma membrane ERK with distinct temporal dynamics

    Jeremiah Keyes, Ambhighainath Ganesan ... Jin Zhang
    An individual extracellular signal regulates multiple cellular actions through differences in the temporal dynamics of spatially distinct populations of the central signaling enzyme, extracellular-signal regulated kinase.
    1. Cell Biology

    Novel repertoire of tau biosensors to monitor pathological tau transformation and seeding activity in living cells

    Erika Cecon, Atsuro Oishi ... Ralf Jockers
    Development of nanoluciferase complementation-based tau biosensors to detect tau conformational change and oligomerization in living cells in high-throughput assays.
    1. Cell Biology
    2. Plant Biology

    Biosensors: Plant biologists FRET over stress

    Won-Gyu Choi, Simon Gilroy
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    1. Cell Biology

    Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium

    Jan N Hansen, Fabian Kaiser ... Dagmar Wachten
    Trafficking of optogenetic tools to primary cilia using nanobodies allows to study cAMP signaling with spatial and temporal resolution independent of the cell body.
    1. Cell Biology

    Single-cell dynamics of pannexin-1-facilitated programmed ATP loss during apoptosis

    Hiromi Imamura, Shuichiro Sakamoto ... Akira Kakizuka
    Cleavage of pannexin-1 channel by caspases facilitates the loss of intracellular ATP level and attenuates glycolytic metabolism of apoptotic cells.
    1. Cell Biology
    2. Computational and Systems Biology

    Removing physiological motion from intravital and clinical functional imaging data

    Sean C Warren, Max Nobis ... Paul Timpson
    Image-based motion correction enables the use of fluorescence lifetime and other functional imaging modalities in an intravital and clinical context in the presence of physiological motion.
    1. Cell Biology

    Conformational specificity of opioid receptors is determined by subcellular location irrespective of agonist

    Stephanie E Crilly, Wooree Ko ... Manojkumar A Puthenveedu
    A GPCR activated by the same agonist engages different conformational biosensors at different subcellular locations, suggesting that location-based conformations drive spatial encoding of signaling.

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