In vitro model of germline specification in marmosets established by directed differentiation of induced pluripotent stem cells, which transcriptionally resemble pre-migratory primordial germ cells in vivo.
Bioinformatics analyses reveal that the Krüppel-associated box domain zinc finger proteins are associated with DNA methylation of retroelements in human primordial germ cells which highlight potential molecular mechanisms for the regulation of retroelements in human male germ cells.
Highly reproducible and efficient human primordial germ cell differentiation was achieved through geometric confinement, which enabled quantitative dissection of the cell signaling driving this differentiation, revealing a major role of Wnt signaling is indirect by inducing Nodal signaling.
Mitsuhiro Endoh, Takaho A Endo ... Haruhiko Koseki
PCGF6 links sequence specific target recognition by the MAX/MGA transcription factor complex to PRC1 (polycomb repressive complex 1) -dependent transcriptional silencing of germ cell-specific genes in mouse pluripotent stem cells.
Cheng-Yi Chen, Sean A McKinney ... Matthew C Gibson
The dependence of Nematostella germ cell specification on zygotic Hedgehog pathway activity supports the hypothesis that the eumetazoan common ancestor segregated its germline by inductive signals rather than maternal determinants.
The lncRNA landscapes in human germ cells are comprehensively profiled and the functional role of an lncRNA LNC1845 in regulating gene expression of a neighboring coding gene LHX8 is elucidated.
Wnt signaling regulates cellular redox in the germline stem cell differentiation niche via a novel regulatory mechanism, thereby controlling germ line stem cell progeny differentiation.
The scaffolding protein Oskar organizes two types of germ granules by phase transition within the same cell but with distinct morphologies, composition and biological functions.
