Lindsay D Clark, Igor Dikiy ... Daniel M Rosenbaum
Generation of a highly deuterated 13C-methyl labeled wild-type GPCR sample is used to facilitate characterization of the molecular environments and fast ps-ns dynamics of sidechains when the receptor is bound to ligands of different efficacy.
Trisha V Vaidyanathan, Max Collard ... Kira E Poskanzer
Cortical astrocytes play key roles in NREM sleep by regulating sleep depth and duration through separate GPCR pathways, and differentially control neuronal slow-wave activity in local and remote cortical circuits.
One-step Isolation and Lysis (OIL) PCR offers a robust, versatile, accessible, and high-throughput method for linking mobile DNA with bacterial hosts in natural microbial communities.
Contrary to previous findings, class A GPCRs share a common activation pathway that directly links ligand binding to G-protein activation, as revealed by novel quantitative analysis.
Alexander S Hauser, Charlotte Avet ... David E Gloriam
The GPCR-G protein coupling map and selectivity insights will catalyze advances in receptor research, cellular signaling, and drug discovery exploiting G protein signaling bias to design safer drugs.
A polarized Moody/protein kinase A pathway plays an unrecognized central role in controlling the enormous cell growth and blood–brain barrier integrity in a highly coordinated spatiotemporal manner in brain homeostasis.
Thibaut Laboute, Jorge Gandía ... Jérôme AJ Becker
GPR88 inhibits G-protein signaling of nearby GPCRs, and dampens b-arrestin recruitment by all GPCRs tested, likely by sequestration in intracellular compartments.
Spatial restriction of GPCR signaling at nascent synapses by adhesion complexes drives excitatory synapse formation and specificity in the hippocampus.
An interdisciplinary approach uncovers a new antinociceptive molecular mechanism and shows that the adhesion GPCR CIRL adjusts the sensation of gentle touch and noxious mechanical insult in opposite directions.