204 results found
    1. Cell Biology

    A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag

    Willa Wen-You Yim, Hayashi Yamamoto, Noboru Mizushima
    HaloTag becomes resistant to lysosomal proteolysis upon ligand binding, enabling us to measure various types of autophagic flux easily and precisely by using HaloTag-containing reporters for processing assays.
    1. Chromosomes and Gene Expression

    RNA Polymerase II cluster dynamics predict mRNA output in living cells

    Won-Ki Cho, Namrata Jayanth ... Ibrahim I Cisse
    Quantitative super resolution imaging, in live mammalian cells, reveals a direct relationship between protein clustering dynamics and the number of mRNA transcribed at an endogenous gene locus.
    1. Cell Biology
    2. Neuroscience

    Synaptic vesicle proteins are selectively delivered to axons in mammalian neurons

    Emma T Watson, Michaela M Pauers ... Edwin R Chapman
    Using carefully controlled expression levels and a variety of optical pulse-chase assays in mammalian neurons, an updated model for the polarized transport of synaptic vesicle proteins, and the first steps of synaptic vesicle biogenesis, were uncovered.
    1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    Systematic analysis of the molecular and biophysical properties of key DNA damage response factors

    Joshua R Heyza, Mariia Mikhova ... Jens C Schmidt
    Live-cell single-molecule imaging of DNA repair factors can be used to analyze their localization to DNA lesions and dissect the hierarchy of their recruitment.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Endoplasmic reticulum stress activates human IRE1α through reversible assembly of inactive dimers into small oligomers

    Vladislav Belyy, Iratxe Zuazo-Gaztelu ... Peter Walter
    Stress sensors in the membrane of the endoplasmic reticulum respond to the accumulation of unfolded proteins by briefly forming small phosphorylation-competent oligomers and dissolving back into active dimers.
    1. Biochemistry and Chemical Biology
    2. Genetics and Genomics

    Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies

    Jun Xu, Ah-Ram Kim ... Norbert Perrimon
    The in vitro and in vivo characterization of two nanobody/NanoTag pairs in the Drosophila system provides a variety of assays for protein detection and manipulation.
    1. Cell Biology

    Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength

    Eike K Mahlandt, Sebastián Palacios Martínez ... Joachim Goedhart
    Optogenetic activation of Rho GTPases provides spatiotemporal control over endothelial cell shape and enables control over endothelial barrier function by manipulating the cell-cell overlap with blue light.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Determining cellular CTCF and cohesin abundances to constrain 3D genome models

    Claudia Cattoglio, Iryna Pustova ... Anders S Hansen
    Absolute quantification of CTCF and cohesin reveals quantitative constraints on 3D genome organization and illuminates the molecular architecture of the cohesin complex.
    1. Cell Biology

    Cell Adhesion-Dependent Biphasic Axon Outgrowth Elucidated by Femtosecond Laser Impulse

    Sohei Yamada, Kentarou Baba ... Yoichiroh Hosokawa
    1. Biochemistry and Chemical Biology
    2. Physics of Living Systems

    Recovering mixtures of fast-diffusing states from short single-particle trajectories

    Alec Heckert, Liza Dahal ... Xavier Darzacq
    Tracking proteins in live cells is challenging due to technical limitations and biological complexity, but approaches based in Bayesian nonparametrics stand a decent chance at recovering a target protein's dynamic profile from noisy data.

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