The in vivo modification of the canonical intermediate filament protein vimentin with O-linked beta-N-acetylglucosamine affects its function in filament assembly, cell migration and host-pathogen interactions.

A newly discovered cytoplasmic intermediate filament in water bears may help these animals to resist extreme environmental conditions.

Aberrant cytoskeletal intermediate filament networks in the C. elegans intestine are associated with hyperphosphorylated intermediate filament proteins and impact not only intestinal cell structure but have also detrimental consequences for organismal well-being.

Mapping intermediate filaments in three dimensions reveals that the organization of these filaments differs across cell types.

The transcription factor Snai1b maintains the myocardial wall integrity by limiting cell extrusions and regulating the intermediate filament gene desmin b.

Specific to muscles and essential for their development, desmin is revealed to be fundamental to the mechanics and shape maintenance of model muscle tissue even in early differentiating and unorganized systems through an integrated magnetic tensiometer approach.

Keratins assemble into tubular filaments with a central density and exhibit structural heterogeneity, which is reflected in alterations of the helical arrangement.

Although intermediate filaments are not widely known to contribute to the morphogenesis of cellular protusions, keratin filaments and cytolinkers are critical for the development of microridge protrusions in zebrafish skin.

Based on 3D visualization of fluorescently labeled cytoskeletal networks, an imaging platform is provided, which allows 3D network reconstruction, immersive visualization and quantification of local network properties serving as blueprints for functional modeling.

TANGO1 functions as a linactant filament to stabilize shallow COPII-coated buds, and after which membrane tension regulation, possibly mediated by TANGO1-controlled membrane fusion, facilitates bud elongation for procollagen export.