5,468 results found
    1. Plant Biology

    Proximity labeling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID

    Andrea Mair, Shou-Ling Xu ... Dominique C Bergmann
    Proximity-labeling using engineered biotin ligases TurboID and miniTurbo enables detection of cell-type-specific and low abundance protein complexes and subcellular proteomes in Arabidopsis and other plants.
    1. Neuroscience

    Microglial motility is modulated by neuronal activity and correlates with dendritic spine plasticity in the hippocampus of awake mice

    Felix Christopher Nebeling, Stefanie Poll ... Martin Fuhrmann
    Microglia that presumably sense neuronal activity via detection of glutamate at synapses in the hippocampus show higher fine process motility and increased contact rates associated with formation and elimination of dendritic spines under conditions of elevated neuronal activity.
    1. Neuroscience

    Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags

    Richi Sakaguchi, Marcus N Leiwe, Takeshi Imai
    A bright and stochastic multicolor labeling method, Tetbow, facilitates millimeters-scale reconstructions of neuronal circuits at a large scale using tissue clearing.
    1. Neuroscience

    A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes

    Réza Shahidi, Elizabeth A Williams ... Gáspár Jékely
    A new technique called ‘siGOLD’ allows neural circuits to be mapped using a combination of antibody labeling and electron microscopy.
    1. Biochemistry and Chemical Biology

    Long-lived metabolic enzymes in the crystalline lens identified by pulse-labeling of mice and mass spectrometry

    Pan Liu, Seby Louis Edassery ... Jing Jin
    By performing 15N pulse-labeling of mice, the turnover of hundreds of proteins in eye tissues was measured by mass spectrometry that revealed long-lived metabolic enzymes in the lens.
    Short Report Updated
    Formats available:
    • HTML
    • PDF
    1. Cell Biology

    Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans

    Ian F Price, Hannah L Hertz ... Wen Tang
    A proximity labeling approach defines germ granule proteome in Caenorhabditis elegans, and identifies LOTUS domain proteins as key regulators of germ granule assembly.
    1. Neuroscience

    Cell-specific non-canonical amino acid labelling identifies changes in the de novo proteome during memory formation

    Harrison Tudor Evans, Liviu-Gabriel Bodea, Jürgen Götz
    Quantitative de novo proteomics paired with in vivo cell-specific non-canonical amino acid labelling identified several spatial long-term memory-induced changes in protein synthesis in hippocampal neurons.
    1. Cell Biology

    Tissue libraries enable rapid determination of conditions that preserve antibody labeling in cleared mouse and human tissue

    Theodore J Zwang, Rachel E Bennett ... Bradley T Hyman
    Conditions for multiplexed antibody labeling in mouse brain tissue translates to effective labeling in human brain tissue when samples are prepared similarly.
    1. Physics of Living Systems

    An in silico testbed for fast and accurate MR labeling of orthopedic implants

    Gregory M Noetscher, Peter J Serano ... Sergey N Makaroff
    Radio frequency heating induced during magnetic resonance procedures within patients that carry embedded passive orthopedic implants may be accurately estimated through modeling and simulation.
    1. Cell Biology

    Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling

    Yoanna Ariosa-Morejon, Alberto Santos ... Tonia L Vincent
    Protein dynamics signatures in ageing collagen-rich tissues that are central to frequent age-related diseases such as osteoporosis and osteoarthritis.

Refine your results by:

Type
Research categories