62 results found
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Phosphatidylinositol 3-kinase and COPII generate LC3 lipidation vesicles from the ER-Golgi intermediate compartment

    Liang Ge, Min Zhang, Randy Schekman
    Building on previous work (Ge et al., 2013), it is shown that the ER-Golgi intermediate compartment is a platform for the production of COPII vesicles as precursor membranes for the lipidation of LC3, which is an essential step in autophagosome biogenesis.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    The ER–Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis

    Liang Ge, David Melville ... Randy Schekman
    A cell-free biochemical assay for protein lipidation identifies the ER–Golgi intermediate compartment as a key early station in the formation of an autophagosome.
    1. Cell Biology

    The ULK1-FBXW5-SEC23B nexus controls autophagy

    Yeon-Tae Jeong, Daniele Simoneschi ... Michele Pagano
    The control of SEC23B degradation by SCFFBXW5 and ULK1 regulates the autophagy.
    1. Cell Biology

    Structural basis for membrane recruitment of ATG16L1 by WIPI2 in autophagy

    Lisa M Strong, Chunmei Chang ... James H Hurley
    The crystal structure of human WIPI2 bound to the ATG16L1 WIPI2-interacting region, combined with in vitro reconstitution and cellular autophagy assays, shows how the LC3 lipidation machinery is recruited in autophagy initiation.
    1. Cell Biology

    LGG-1/GABARAP lipidation is not required for autophagy and development in Caenorhabditis elegans

    Romane Leboutet, Céline Largeau ... Renaud Legouis
    A genetic approach in Caenorhabditis elegans shows that the cleaved and cytosolic form of the ubiquitin-like protein LGG-1/GABARAP is sufficient for both autophagy and embryonic development.
    1. Immunology and Inflammation

    Autophagy is a critical regulator of memory CD8+ T cell formation

    Daniel J Puleston, Hanlin Zhang ... Anna Katharina Simon
    Autophagy is required for CD8+ T cell memory formation; in aged individuals autophagy levels are diminished, and increasing autophagy enhances their CD8+ T cell responses.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Autophagosome membrane expansion is mediated by the N-terminus and cis-membrane association of human ATG8s

    Wenxin Zhang, Taki Nishimura ... Sharon A Tooze
    cis-membrane insertion of human ATG8 N-terminus facilitates autophagosome membrane expansion independent of autophagic cargo.
    1. Cell Biology

    Spatiotemporal regulation of autophagy during Caenorhabditis elegans aging

    Jessica T Chang, Caroline Kumsta ... Malene Hansen
    Autophagic flux assays in the nematode Caenorhabditis elegans suggest that autophagy decreases during normal aging, whereas long-lived daf-2 and glp-1 mutants maintain autophagic capacity in distinct spatiotemporal-specific manners to extend lifespan.
    1. Cell Biology
    2. Neuroscience

    Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons

    Andrea KH Stavoe, Pallavi P Gopal ... Erika LF Holzbaur
    The dynamic phosphorylation of autophagy component WIPI2B is critical to maintain autophagosome biogenesis in neurons, and ectopic expression of WIPI2B can restore rates of autophagosome biogenesis in aged neurons.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Elucidation of the anti-autophagy mechanism of the Legionella effector RavZ using semisynthetic LC3 proteins

    Aimin Yang, Supansa Pantoom, Yao-Wen Wu
    Legionella effector RavZ evades host autophagy by extracting LC3-PE from the membrane before deconjugation.

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