Confronting different models of chromatin accessibility with temporally resolved transcription profiles favors a scenario where transcription factors actively, rather than passively, drive chromatin from the inaccessible to the accessible state.
The transcription machinery is required for the disassembly of the promoter-proximal H2A.Z nucleosome, contributing to the constitutive histone turnover at yeast promoters.
Andrea Corsinotti, Frederick CK Wong ... Ian Chambers
Genetic manipulations show that endogenous transcription factors of the SoxB1 class act redundantly to maintain primed pluripotency and reveal differential effects on transitions between pluripotent and differentiation states.
Evolution-guided structure-function analyses reveal that CD4 transmembrane and intracellular juxtamembrane motifs play a more profound role in initiating pMHCII-specific TCR-CD3 signaling than previously characterized CD4-Lck interactions.
Confidence-dependent reinforcement learning is active and produces trial-to-trial choice updating even in well-learned perceptual decisions without explicit reward biases, across species and sensory modalities.
Evelyn L Eastwood, Kayla A Jara ... Gregory J Hannon
Heterochromatin formation at transposon loci depends on dimerisation of the effector complex that elicits co-transcriptional silencing and this requirement is fulfilled by co-option of the conserved dimerisation hub protein, Cut-up/LC8.
A newly described isolate of the behavior-manipulating fly pathogen Entomophthora muscae that naturally infects fruit flies can be cultured in the lab, enabling molecular investigation of how microbes induce behavior changes in animal hosts.
An in vivo disulfide crosslinking assay shows preferential disassembly of nucleosomes with two H2A.Z histones by transcription machinery in yeast and conjugation to one or two ubiquitin moieties in human cells.
