Yujia Cai, Anders Laustsen ... Jacob Giehm Mikkelsen
Building on previous work (Cai et al., 2014), HIV-derived lentiviral vectors are harnessed to insert user-defined genes into a safe locus in the genome of human stem cells.
Lentiviral protein transduction offers a new approach for administration of custom-designed nucleases, leading to high-efficacy targeted gene editing and reduced off-target activity after virus-directed delivery of programmable nuclease proteins.
Host restriction factors that block cross-species transmission also play a role in limiting the replication of highly-adapted HIV-1 in IFN-stimulated cells.
Haritha Mathsyaraja, Jonathen Catchpole ... Robert N Eisenman
The MYC transcription factor network member MGA is a subunit of a non-canonical Polycomb complex, which, when inactivated, accelerates tumorigenesis in mouse models of cancer and proliferation in colon organoids.
Plasmodium parasites use host-derived factors to form more rosettes and hamper phagocytosis, representing a new escape mechanism for the malaria parasites.
Gαq stimulates HDAC5 phosphorylation by PKC-independent PKD/PKCμ activation to induce the IκB expression and limit the NF-κB-mediated inflammatory response which essential for proper endometrial decidualization and early pregnancy.
Oct1 replaces Oct4 at critical targets shortly after ESC differentiation to promote expression of lineage-specific genes and repress lineage-inappropriate genes.