61 results found
    1. Structural Biology and Molecular Biophysics

    Fidaxomicin jams Mycobacterium tuberculosis RNA polymerase motions needed for initiation via RbpA contacts

    Hande Boyaci, James Chen ... Elizabeth A Campbell
    Cryo-electron microscopy structures show how the clinically used antimicrobial fidaxomicin binds and inhibits Mycobacterium tuberculosis RNA polymerase by acting like a doorstop to jam the enzyme in an open conformation via the general transcription factor RbpA.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Arrayed CRISPRi and quantitative imaging describe the morphotypic landscape of essential mycobacterial genes

    Timothy J de Wet, Kristy R Winkler ... Digby F Warner
    A high-throughput functional genomics approach combining inducible CRISPR-interference and quantitative imaging yields an atlas of 'phenoprints' to guide gene function assignments, identify metabolic pathway-specific morphotypes, and inform antibiotic mechanism-of-action studies.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structure and function of the mycobacterial transcription initiation complex with the essential regulator RbpA

    Elizabeth A Hubin, Allison Fay ... Elizabeth A Campbell
    The essential mycobacterial transcription factor RbpA interacts with promoter DNA and cooperates with another essential transcription factor, CarD, to stimulate the formation of an intermediate leading to the open promoter complex.
    1. Biochemistry and Chemical Biology

    Membrane-partitioned cell wall synthesis in mycobacteria

    Alam García-Heredia, Takehiro Kado ... M Sloan Siegrist
    Mycobacteria employ plasma membrane compartments to organize their cell wall synthesis, and the finalized cell wall compartmentalizes the plasma membrane to promote an environment conducive to its own synthesis.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Investigating the composition and recruitment of the mycobacterial ImuA′–ImuB–DnaE2 mutasome

    Sophia Gessner, Zela Alexandria-Mae Martin ... Digby F Warner
    The interaction of ImuB with the β sliding clamp is essential for induced mutagenesis in mycobacteria and could be a novel target for new anti-tuberculosis drugs designed to inhibit the emergence of genetic resistance.
    1. Microbiology and Infectious Disease

    A single regulator NrtR controls bacterial NAD+ homeostasis via its acetylation

    Rongsui Gao, Wenhui Wei ... Youjun Feng
    Functional definition of NrtR and the discovery of its acetylation represents a first paradigm for linking protein acetylation to bacterial central NAD+ metabolism.
    1. Microbiology and Infectious Disease

    Kasugamycin potentiates rifampicin and limits emergence of resistance in Mycobacterium tuberculosis by specifically decreasing mycobacterial mistranslation

    Swarnava Chaudhuri, Liping Li ... Babak Javid
    Kasugamycin potentiates rifampicin killing of Mycobacterium tuberculosis by targeting adaptive mistranslation.
    1. Microbiology and Infectious Disease

    Pervasive translation in Mycobacterium tuberculosis

    Carol Smith, Jill G Canestrari ... Joseph T Wade
    Thousands of novel open-reading frames (ORFs) are translated in the bacterium Mycobacterium tuberculosis, including many short ORFs that are likely to contribute to cell fitness.
    1. Microbiology and Infectious Disease

    Mycobacterium tuberculosis SatS is a chaperone for the SecA2 protein export pathway

    Brittany K Miller, Ryan Hughes ... Miriam Braunstein
    SatS of Mycobacterium tuberculosis is a new protein export chaperone with a role in exporting proteins by the specialized SecA2 pathway and a role in intracellular growth in macrophages.
    1. Structural Biology and Molecular Biophysics

    Structure of Mycobacterium tuberculosis cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

    Shan Zhou, Weiwei Wang ... Hongri Gong
    The three-dimensional structures of Mycobacterium tuberculosis cytochrome bcc in complex with the antituberculosis agents, Q203 and TB47, explain how these inhibitors suppress activity of the complex.

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