A community-based international effort generates standardized HLA allele-specific peptide assay libraries and enables the analysis of the human immunopeptidome by SWATH mass spectrometry.
By performing 15N pulse-labeling of mice, the turnover of hundreds of proteins in eye tissues was measured by mass spectrometry that revealed long-lived metabolic enzymes in the lens.
A new protocol step improves robustness and ease-of-use for mass spectrometry in the clinic, opening the door to mass deployment to monitor infectious agents.
Dipali G Sashital, Candacia A Greeman ... James R Williamson
Comprehensive structural and compositional characterization of the in vivo 30S ribosomal assembly landscape reveals parallel pathways for 3′-domain formation and a previously unknown role for the assembly factor RimP.
Mass spectrometry exposes a post-transcriptionally regulated reduction in protein diversity in hematopoietic stem cells, including a lack of detectable Dnmt3a protein levels despite mRNA levels comparable to progenitors.
Deepti Karandur, Moitrayee Bhattacharyya ... John Kuriyan
Activation and autophosphorylation of CaMKII releases the regulatory segment, which can then bind to and destabilize the hub assembly by trapping large fluctuations in the hub architecture.
Labeling with 13C and 15N in the absence of metabolic engineering enabled the exploration of peptidoglycan metabolism at a very fine level of detail based on kinetic characterization of isotopologues predicted to occur according to known recycling and biosynthesis pathways.
Ma Teresa Pellicer Martinez, Jason C Crack ... Nick E Le Brun
Molecular details of how the iron–sulfur cluster cofactor of a bacterial global iron regulatory protein simultaneously senses iron and O2 are revealed by mass spectrometry and spectroscopy.