Many experimentally identified microRNA-binding sites are ineffective at mediating repression, and an improved quantitative model for predicting the effective sites is as informative as high-throughput experimental approaches.

Argonaute proteins directly contact adenosine nucleotides in some miRNA target sites, leading to enhanced repression of the target mRNA.

Affinity measurements of microRNA sites representing >1000 different pairing architectures reveal that two binding modes can mediate pairing to the microRNA 3′ region, and that microRNA G and oligo(G/C) residues are most impactful.

Primate-specific miR-934 mediates neurogenesis and downstream neuronal differentiation processes, modulating the expression of genes associated with the subplate, a region most prominent in primates, emerging during early cortical development.

A single microRNA-binding site in a long non-coding RNA alters T cell responses in vivo by 'sponging' the miRNA to inhibit its gene regulatory function.

Repressing the conserved pro-differentiation let-7 microRNAs through limiting Ago2 levels is critical to maintaining pluripotency in stem cells.

Human plasma contains protein-protected mRNA fragments, myriad repeat RNAs, and novel intron RNAs, including a family of structured full-length excised introns, some corresponding to mirtron pre-miRNAs and agotrons.

iRICC, a new method to identify RNA-RNA interactions in vivo, provides mechanistic insight into the function of a viral non-coding RNA.

In isogenically matched colorectal cancer (CRC) cell lines, mutant KRAS alters the composition of secreted miRNAs in extracellular vesicles that can then transfer repressive activity to wild type cells.

The central RNA interference protein, human Argonaute-2, releases its target following phosphorylation on a critical loop in the protein, freeing microRNA-bound Argonaute-2 to seek out and repress additional targets.