A sophisticated classical mutagenesis assay with next-generation sequencing technology presents a benefit to future research on mutagenesis, DNA repair, and cancer.

Deep mutagenesis experiments demonstrate that while cancer-causing mutations in Ras selectively alter regulatory interactions with GTPase-activating proteins, Ras is also activated by many mutations that decrease protein stability.

Genetic and biochemical analyses reveal an important role for the translesion DNA polymerase DinB2 in mycobacterial mutagenesis, including in the insertion/deletion events that are an increasingly recognized type of diversity in Mycobacterium tuberculosis genomes.

Experimental determination of residue contacts from mutational data allows model discrimination and identification of in vivo functional conformations of proteins.

Genome-scale integration of transposon mutagenesis with a redox biosensor identified a hypothetical transcription factor- Rv0158 required to calibrate the growth, cytoplasmic redox potential, and respiration of Mycobacterium tuberculosis in response to metabolic switching from glucose to fatty acids.

Favipiravir, a novel nucleoside analogue, can clear a persistent norovirus infection in vivo through lethal mutagenesis.

ENU mutagenesis in pigs is an efficient strategy to introduce mutations at the whole-genome level and mutants have been generated on a large scale for agricultural production and biomedical research.

Deep mutagenesis reveals that mutations rarely alter the inclusion of highly-included exons.

Measurement of the effect of all single-codon variants of the AAV2 rep gene on recombinant adeno-associated virus production identifies novel beneficial variants, which may facilitate improvement of gene therapy production.

A large-scale CRISPR library allows efficient gene disruption with spatial and temporal control in Drosophila.