Nanobodies are developed that target neuronal proteins with restricted subcellular distributions and used as intrabodies to deliver cargo to specific sites within neurons and nanoscale immunolabels for improved imaging resolution.
Jonathan CY Tang, Eugene Drokhlyansky ... Constance L Cepko
A straightforward, rapid and generalizable strategy uses modified nanobodies to convert intracellular protein detection into desired biological activities in cells and animals.
A collection of 22 nanobodies against 11 different Drosophila sarcomeric protein epitopes was generated, validated in stainings and for some transgenic fly lines were generated to monitor the dynamics of the sarcomeric proteins in muscles in vivo.
A large repertoire of nanobodies that target discrete regions of SARS-CoV-2 spike shows effective neutralization against variants of concern with many pairwise combinations resistant to escape and demonstrating synergistic neutralization activities.
John G Dingus, Jonathan CY Tang ... Constance L Cepko
A large proportion of nanobodies are unstable when expressed intracellularly, but can be reliably stabilized for intracellular expression by applying a generalizable mutagenesis strategy based on positional sequence conservation.
Florian Schueder, Pierre Mangeol ... Frank Schnorrer
The two Drosophila titin homologs Sallimus and Projectin display a precise tandem arrangement in the sarcomere that may explain how sarcomere length is regulated outside of vertebrates.
The in vitro and in vivo characterization of two nanobody/NanoTag pairs in the Drosophila system provides a variety of assays for protein detection and manipulation.
A fully synthetic library of humanized single domain antibodies yields in vitro high affinity antibodies usable in cell biology and translational projects.
Karthik Ramanadane, Monique S Straub ... Cristina Manatschal
The structural and functional characterization of EleNRMT defines the basis for the distinct ion selectivity of a clade of the SLC11/NRAMP family which, instead of transporting transition metal ions, have evolved as uncoupled transporters of Mg2+.