88 results found
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    The Ndc80 complex bridges two Dam1 complex rings

    Jae ook Kim, Alex Zelter ... Trisha N Davis
    The ability of the Ndc80 complex to bind two Dam1 complex rings is important for chromosome attachment, and the distance between the two rings is vital for supporting growth.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Multivalency of NDC80 in the outer kinetochore is essential to track shortening microtubules and generate forces

    Vladimir A Volkov, Pim J Huis in 't Veld ... Andrea Musacchio
    Multiple NDC80 complexes in the outer kinetochore cooperate to bind the ends of depolymerizing microtubules and harness their power stroke.
    1. Cell Biology

    Measuring NDC80 binding reveals the molecular basis of tension-dependent kinetochore-microtubule attachments

    Tae Yeon Yoo, Jeong-Mo Choi ... Daniel J Needleman
    Quantitative experiments and theory show that the tension-dependent regulation of NDC80 binding to kinetochore microtubules arises from a combination of the changing Aurora B concentration at NDC80 and the nonlinearity of Aurora B autoactivation.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Structural basis of Stu2 recruitment to yeast kinetochores

    Jacob A Zahm, Michael G Stewart ... Matthew P Miller
    The results here show that Stu2 binds kinetochores by associating with the Ndc80 complex and that the interaction is critical for accurate chromosome segregation during cell division.
    1. Chromosomes and Gene Expression

    Transcription of a 5’ extended mRNA isoform directs dynamic chromatin changes and interference of a downstream promoter

    Minghao Chia, Amy Tresenrider ... Folkert Jacobus van Werven
    Expression of a 5' extended mRNA isoform represses transcription of a downstream promoter.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Tight bending of the Ndc80 complex provides intrinsic regulation of its binding to microtubules

    Emily Anne Scarborough, Trisha N Davis, Charles L Asbury
    Flexibility of Ndc80 provides an inherent mode of regulation for the conserved kinetochore complex, unique from well-studied phospho-regulation.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Molecular determinants of the Ska-Ndc80 interaction and their influence on microtubule tracking and force-coupling

    Pim J Huis in 't Veld, Vladimir A Volkov ... Marileen Dogterom
    Binding of two macromolecular complexes allows kinetochores to capture force produced by the depolymerising ends of microtubules, allowing chromosomes to be transmitted from a mother cell to its two daughters.
    1. Chromosomes and Gene Expression

    Kinetochore inactivation by expression of a repressive mRNA

    Jingxun Chen, Amy Tresenrider ... Elçin Ünal
    Rather than being a protein coding unit, an mRNA molecule can serve a purely regulatory function to inhibit protein synthesis of its corresponding gene.
    1. Biochemistry and Chemical Biology

    An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast

    Jackie Lang, Adrienne Barber, Sue Biggins
    An assay to assemble kinetochores de novo was developed and used to identify the role of the CENP-T pathway.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Molecular basis of outer kinetochore assembly on CENP-T

    Pim J Huis in 't Veld, Sadasivam Jeganathan ... Andrea Musacchio
    The centromeric protein CENP-T assembles the microtubule-binding interface of kinetochores through direct recruitment of two Ndc80 complexes and indirect recruitment of a third one through the Mis12 complex.

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