Automated liquid handling, whole mount staining, and clearing allow unbiased 3D quantitation of cell markers in human neural organoids with diameters of up to 1 mm at the single-cell level.

Human somites are formed in organoids from human pluripotent stem cells.

Skeletal muscle organoids differentiated from human pluripotent stem cells offer a system for investigating myogenesis and satellite cell development with translational potential for muscular dystrophy modeling and therapy development.

Human liver organoids provide a patient-derived platform to interrogate host and viral mechanisms of HBV replication, perform anti-HBV and toxicity drug screens, and investigate the molecular determinants of related tumorigenesis.

Kidney organoids are a high-fidelity system for investigating basement membrane regulation in development and disease.

Label-free three-photon imaging of whole, uncleared intact organoids generated from Rett syndrome patients show shorter migration distances and slower migration speeds of mutant radially migrating neurons with more tortuous trajectories.

Directed differentiation of stem cells can generate ventral-anterior foregut spheroids that can expand into three-dimensional lung organoids with striking structural, cellular and molecular similarities to the human fetal lung.

Organoids developed from matched human placental tissue define differences in antiviral signaling between cell types comprising the maternal-fetal interface.

A vessel and brain fusion organoid model provides a platform for the study of interactions between neuronal and non-neuronal components during brain development and functioning.

Fifteen continuous high-grade serous ovarian carcinoma patient-derived organoids are characterized by transcriptomic, genomic, and drug sensitivity assays to reveal that they comprise communities of clonal populations and represent models of different causes of chromosomal instability and degrees of genome complexity.