Iwan Zimmermann, Pascal Egloff ... Markus A Seeger
Synthetic single domain antibody libraries and a binder selection cascade encompassing ribosome and phage display enable the selection of conformation-specific binders against previously intractable membrane proteins within three weeks.
A fully synthetic library of humanized single domain antibodies yields in vitro high affinity antibodies usable in cell biology and translational projects.
Madhura Raghavan, Katrina L Kalantar ... Joseph L DeRisi
The dominant targets of host immune response to malaria are repeat elements, which are short stretches of amino acids repeated within Plasmodium falciparum proteins, and these are associated with short-lived and exposure-dependent antibody responses.
Cryo electron microscopy and structure-based mutagenesis reveal that the bacteriophage BPP-1 contains two of the three major recognized viral folds, one of which exhibits a new topology.
Sara E Vazquez, Elise MN Ferré ... Mark S Anderson
Humans with mutations in the AIRE gene exhibit common autoantibodies targeting ovarian and intestinal antigens, including intestinal dysfunction-associated antibodies to enteroendocrine transcription factor RFX6.
The coupling of the enzyme/structure core to different short linear motifs represents a novel mechanism to diversify and expand the function of signaling proteins.
Huy Quoc Nguyen, Jagoree Roy ... Polly Morrell Fordyce
A novel high-throughput method for measuring many weak protein-peptide affinities simultaneously reveals how calcineurin, a human phosphatase essential for the immune response, recognizes its peptide substrates.
Angela M Phillips, Katherine R Lawrence ... Michael M Desai
CR9114, one of the most broadly neutralizing anti-influenza antibodies characterized to date, acquires affinity to divergent HA subtypes sequentially, due to higher order interactions between the nested sets of mutations required for binding each distinct subtype.