Combining ribosome profiling and RNA sequencing illuminates novel features of porcine reproductive and respiratory syndrome virus gene expression, including the regulation of polyprotein stoichiometry through temporal modulation of ribosomal frameshifting and the synthesis of non-canonical transcripts.

With the removal of confounding artifacts, ribosome profiling can yield insight into the mechanism of protein synthesis in bacteria at high resolution.

Ribosomes translate through stop codons far more often than previously thought, yielding C-terminally extended proteins in a variety of eukaryotes.

Profilin release from the filament end controls the speed of actin growth at physiological conditions.

Profilin helps to maintain formin at the growing barbed end of an actin filament, while piconewton mechanical tension drastically enhances formin dissociation from the barbed end.

Gene function can be revealed based on characteristic phenotypes using a Cell Painting microscopy assay.

Brain region-specific profiling of O-GlcNAcylation interactome in Drosophila mushroom body suggests that hypo-O-GlcNAcylation impacts cognitive function by regulating translational activity.

Fluorescently-tagged profilin-1 behaves similar to the tag-free protein in biochemical and cell-based assays.

Enhancements to the CUT&RUN antibody-targeted nuclease strategy for chromatin profiling include an improved affinity-cleavage fusion protein and digestion protocol, and a simplified calibration strategy.

A new luciferase and fluorescent reporter system enables rapid and efficient in-cell profiling of the majority of protein kinase oncogenes known to date.