By fusing influenza viral conserved epitopes and a superantigen fragment, we constructed a recombinant protein that might be a candidate universal broad-spectrum vaccine for the prevention and treatment of multiple influenza viruses.
Ines Heyndrickx, Kim Deswarte ... Bart N Lambrecht
Protein crystallization is a rare event yet Ym1 crystals made from a chitinase-like protein are abundantly found in eosinophilic airway inflammation, where they promote type 2 immunity.
Camila Tenorio França, Michael T White ... Ivo Mueller
Antibody responses to individual and optimal combinations of P. vivax recombinant proteins in naturally-exposed populations help to identify correlates of protective immunity, and establish a clear path to testing a multicomponent P. vivax vaccine.
Matthew E Cockman, Kerstin Lippl ... Peter J Ratcliffe
Assays using recombinant HIF prolyl hydroxylases did not support hydroxylation of more than 20 reported non-HIF substrates under conditions where robust HIF hydroxylation was observed.
Keith F DeLuca, Jeanne E Mick ... Jennifer G DeLuca
Tools and methodologies to create and diversify recombinant antibodies and antibody fragments provide researchers with the ability to generate low-cost, high-yield immunological reagents.
Meng Zhao, Niels Banhos Danneskiold-Samsøe ... Katrin J Svensson
The role of Isthmin-1 in muscle function is defined by using phosphoproteomics to identify distinct and overlapping signaling pathways between Isthmin-1 and insulin.
Nicolas P Andrews, Justin X Boeckman ... James S Trimmer
This paper describes an effective pipeline for conversion of conventional monoclonal antibodies into recombinant form that results in an IgG subclass switch that greatly expands their utility for multiplex labeling.
Ching-Lin Hsieh, Christopher P Ptak ... Yung-Fu Chang
The antibody accessibility of the leptospiral surface protein, LigB, provides a guide for the rational design of improved recombinant chimeric vaccine antigens displayed on a single domain scaffold.
Human cullin-RING ligases are buffered to a much greater extent than had been previously appreciated, and the roles of ubiquitin chain extension enzymes are far more nuanced at physiological concentrations.