Jacob S Lewis, Lisanne M Spenkelink ... Antoine M van Oijen
The canonical model that the bacterial replisome tightly holds on to its polymerases is challenged by the visualization of rapid holoenzyme exchange, both in vitro and in vivo.
Reconstitution of orientation-specific R-loop-replisome collisions with purified proteins reveals the differential impact of R-loop-associated RNA:DNA hybrids and G-quadruplexes on replication fork progression.
Biochemical data demonstrate an unexpected and critical function for the enigmatic Mcm10 protein in helping the eukaryotic CMG helicase/replisome bypass roadblocks on the DNA that may also explain Mcm10 function at origins of replication.
The speed of replisomes in Escherichia coli depends on temperature and varies along the genome in a wave-like manner, with implications for bacterial physiology.
Thomas R Beattie, Nitin Kapadia ... Rodrigo Reyes-Lamothe
DnaB Helicase is the only stable component of the bacterial replisome as the replicative DNA polymerase frequently exchanges in both leading and lagging strands.
Maruthi Kumar Pabba, Christian Ritter ... M Cristina Cardoso
Live cell tracking of labeled DNA reveals that replisome loading rather than DNA synthesis decreases the chromatin motion during S-phase in human cells.
The TRAIP ubiquitin ligase is required during mitosis to disassemble the replisome at sites of incomplete DNA replication, and activate the mitotic DNA repair pathway, thus preserving genome integrity.