2,447 results found
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    E. coli TraR allosterically regulates transcription initiation by altering RNA polymerase conformation

    James Chen, Saumya Gopalkrishnan ... Seth A Darst
    Cryo-electron microscopy structures, combined with biochemical experiments, show how the E. coli F element-encoded TraR protein regulates transcription initiation by altering RNA polymerase conformation and conformational heterogeneity.
    1. Developmental Biology
    2. Chromosomes and Gene Expression

    A global change in RNA polymerase II pausing during the Drosophila midblastula transition

    Kai Chen, Jeff Johnston ... Julia Zeitlinger
    The first zygotic genes are transcribed without RNA polymerase II pausing from a TATA-enriched promoter.
    1. Genetics and Genomics

    Ssl2/TFIIH function in transcription start site scanning by RNA polymerase II in Saccharomyces cerevisiae

    Tingting Zhao, Irina O Vvedenskaya ... Craig D Kaplan
    Ssl2 processivity determines the window for transcription start site selection during promoter scanning by RNA polymerase II in Saccharomyces cerevisiae.
    1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    The cryo-EM structure of a 12-subunit variant of RNA polymerase I reveals dissociation of the A49-A34.5 heterodimer and rearrangement of subunit A12.2

    Lucas Tafur, Yashar Sadian ... Christoph W Müller
    Cryo-EM structures of RNA polymerase I reveal considerable 'transformers-like' rearrangements where one subcomplex dissociates and is replaced by one domain of another subunit, possibly as an additional layer of transcriptional control.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    GE23077 binds to the RNA polymerase ‘i’ and ‘i+1’ sites and prevents the binding of initiating nucleotides

    Yu Zhang, David Degen ... Richard H Ebright
    The cyclic-peptide antibiotic GE23077 inhibits bacterial RNA polymerase through a novel target that exhibits low susceptibility to target-based resistance and that enables synthesis of bipartite inhibitors that are exceptionally potent and refractory to target-based resistance.
    1. Structural Biology and Molecular Biophysics

    Structural mechanism of ATP-independent transcription initiation by RNA polymerase I

    Yan Han, Chunli Yan ... Yuan He
    Structures of RNA polymerase I transcription machinery revealed a ratcheting motion within the complex in coordination with three distinct functional states, implicating a novel mechanism for promoter bubble opening in the absence of ATP hydrolysis.
    1. Chromosomes and Gene Expression

    The dynamic assembly of distinct RNA polymerase I complexes modulates rDNA transcription

    Eva Torreira, Jaime Alegrio Louro ... Carlos Fernández-Tornero
    Live-cell imaging, genetic analysis and electron cryomicroscopy identify structural motifs involved in the differential assembly of Pol I-Rrn3 complexes and Pol I homodimers in response to nutrient availability.
    1. Structural Biology and Molecular Biophysics

    Fidaxomicin jams Mycobacterium tuberculosis RNA polymerase motions needed for initiation via RbpA contacts

    Hande Boyaci, James Chen ... Elizabeth A Campbell
    Cryo-electron microscopy structures show how the clinically used antimicrobial fidaxomicin binds and inhibits Mycobacterium tuberculosis RNA polymerase by acting like a doorstop to jam the enzyme in an open conformation via the general transcription factor RbpA.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex

    Jin Young Kang, Paul Dominic B Olinares ... Seth A Darst
    Cryo-electron microscopy structures show how coliphage HK022 Nun blocks Escherichia coli RNA polymerase translocation by mediating multiple interactions between the RNA polymerase and nucleic acids.
    1. Microbiology and Infectious Disease

    RNA polymerase mutations cause cephalosporin resistance in clinical Neisseria gonorrhoeae isolates

    Samantha G Palace, Yi Wang ... Yonatan H Grad
    Ceftriaxone resistance has arisen multiple times in clinical gonococcal populations via previously undescribed RNA polymerase mutations, underscoring the importance of continued surveillance for novel resistance determinants.

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