In vivo single-molecule imaging demonstrates that Smc5/6 chromatin association depends on ATP hydrolysis and dsDNA binding, requires Nse6 and is modulated by Brc1 after DNA damage.

An open-source user-friendly toolbox implementing machine learning for single-molecule FRET analysis enabling experts and non-experts to reproducibly provide dynamic structural biology insights.

Arc-shaped PFO oligomers consisting of at least four subunits can insert into the membrane in a kinetically controlled process opening a transmembrane pore, whereby post-insertion growth may ultimately lead to formation of a complete ring.

Mars provides a software platform for the development of complex single-molecule analysis workflows enabling the discovery and reporting of new biological phenomena with an open data format accessible to everyone.

Proximity-assisted photoactivation (PAPA) provides a new way to detect protein–protein interactions in single-molecule imaging of live cells.

Upon DNA damage, Rad52 forms a membraneless sub-compartment where Rad52 molecules are highly dynamic and share properties with liquid-liquid phase-separated molecules, reflecting the existence of a liquid droplet around damaged DNA.

A new analysis algorithm (DISC) enables accurate analysis of data from high-throughput single-molecule paradigms and reveals a non-cooperative binding mechanism of cyclic nucleotide-binding domains from HCN ion channels.

A novel single-molecule sequencing method reveals previously unappreciated heterogeneity in nucleosome positioning on individual chromatin fibers across the human genome.

The primer extension and 5' flap processing activities of DNA polymerase I are physically coordinated by combined movements of the DNA substrate and the 5' nuclease domain of the enzyme.

A physics-based, statistically rigorous mathematical model enables automated, objective interpretation of images from single-molecule fluorescence colocalization microscopy experiments.