1,794 results found
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Bayesian machine learning analysis of single-molecule fluorescence colocalization images

    Yerdos A Ordabayev, Larry J Friedman ... Douglas L Theobald
    A physics-based, statistically rigorous mathematical model enables automated, objective interpretation of images from single-molecule fluorescence colocalization microscopy experiments.
    1. Neuroscience

    Tracking multiple conformations occurring on angstrom-and-millisecond scales in single amino-acid-transporter molecules

    Yufeng Zhou, John H Lewis, Zhe Lu
    The present fluorescence polarization microscopy method enables the determination of the spatial orientations and lifetimes of a transporter protein in individual conformational states required for understanding the complex conformational mechanism underlying its function, applicable to investigation of other membrane proteins.
    1. Microbiology and Infectious Disease

    NSF-mediated disassembly of on- and off-pathway SNARE complexes and inhibition by complexin

    Ucheor B Choi, Minglei Zhao ... Axel T Brunger
    NSF is part of a membrane trafficking quality control system that disassembles both properly formed and off-pathway SNARE complexes, and the disassembly activity may be regulated by complexin.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Cellular encoding of Cy dyes for single-molecule imaging

    Lilia Leisle, Rahul Chadda ... Christopher A Ahern
    Cyanine fluorophores are encoded as non-canonical amino acids to produce functional proteins in cell-free translation systems and live cells for single-molecule imaging.
    1. Computational and Systems Biology
    2. Structural Biology and Molecular Biophysics

    Conformational dynamics in TRPV1 channels reported by an encoded coumarin amino acid

    Ximena Steinberg, Marina A Kasimova ... Sebastian E Brauchi
    Single-molecule measurement of conformational dynamics using a genetically encoded fluorescent probe suggests that the selectivity filter region of TRPV1 channels undergoes dynamic motion during agonist activation.
    1. Chromosomes and Gene Expression

    Direct observation of frequency modulated transcription in single cells using light activation

    Daniel R Larson, Christoph Fritzsch ... Robert H Singer
    Steroid hormone receptors control the expression of their target genes through a digital on-off switch in individual cells, which leads to an analogue dose-response relationship at the level of the whole organism.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Multi-step recognition of potential 5' splice sites by the Saccharomyces cerevisiae U1 snRNP

    Sarah R Hansen, David S White ... Aaron A Hoskins
    The yeast U1 snRNP recognizes multiple features of target RNAs to reversibly identify splicing-competent 5' splice sites.
    1. Structural Biology and Molecular Biophysics

    Integrative dynamic structural biology unveils conformers essential for the oligomerization of a large GTPase

    Thomas-O Peulen, Carola S Hengstenberg ... Christian Herrmann
    Multimodal spectroscopy (smFRET, EPR, SAXS, and SANS) and integrative structural modeling reveal large-scale domain rearrangements in human guanylate binding protein 1 (hGBP1) that are driving forces for the formation of oligomers that enable its biological function in innate immune defense.
    1. Structural Biology and Molecular Biophysics

    Nanoscopic anatomy of dynamic multi-protein complexes at membranes resolved by graphene-induced energy transfer

    Nadia Füllbrunn, Zehao Li ... Changjiang You
    The axial organization and dynamics of the HOPS complex at membrane surface are resolved by graphene-induced energy transfer with subnanometer resolution.
    1. Structural Biology and Molecular Biophysics

    Structural dynamics determine voltage and pH gating in human voltage-gated proton channel

    Shuo Han, Sophia Peng ... Shizhen Wang
    Visualization of the real-time conformational transitions of the human voltage-gated proton channel hHv1 provided novel insights into how voltage and pH gradients modify the dynamic behaviors of channel structures to control proton flow across membrane.

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