1,970 results found
    1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    Robust model-based analysis of single-particle tracking experiments with Spot-On

    Anders S Hansen, Maxime Woringer ... Xavier Darzacq
    Spot-On is an easy-to-use website that makes a rigorous and bias-corrected modeling framework for analysis of single-molecule tracking experiments available to all.
    1. Structural Biology and Molecular Biophysics

    Cryo-EM single particle analysis with the Volta phase plate

    Radostin Danev, Wolfgang Baumeister
    The Volta phase plate enables in-focus single particle analysis at near atomic resolutions, which could expand the capabilities of cryo-EM, especially for small, flexible or heterogeneous macromolecular samples.
    1. Structural Biology and Molecular Biophysics

    Using the Volta phase plate with defocus for cryo-EM single particle analysis

    Radostin Danev, Dimitry Tegunov, Wolfgang Baumeister
    Building on previous work in cryo-electron microscopy (Entchev et al, 2015), it shown that a combination of the Volta phase plate and a small amount of defocus can simplify the experimental set-up, increase the data acquisition rate and improve resolution.
    1. Structural Biology and Molecular Biophysics

    Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud

    Michael A Cianfrocco, Andres E Leschziner
    A freely available software environment on Amazon's cloud computing network makes processing of cryo-EM data accessible and affordable.
    1. Structural Biology and Molecular Biophysics

    Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION

    Takanori Nakane, Dari Kimanius ... Sjors HW Scheres
    Multi-body refinement in RELION allows high-resolution reconstruction and visualisation of molecular motions in cryo-EM single-particle data sets with continuous forms of structural heterogeneity.
    1. Structural Biology and Molecular Biophysics

    Beam-induced motion correction for sub-megadalton cryo-EM particles

    Sjors HW Scheres
    Building on previous work (Bai et al., 2013), we describe an algorithm that allows cryo-EM structure determination to near-atomic resolution for protein complexes as small as 170 kDa.
    1. Structural Biology and Molecular Biophysics

    A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0

    Jasenko Zivanov, Joaquín Otón ... Sjors HW Scheres
    New functionality in RELION-4.0 allows convenient structure determination from cryo electron tomography data to resolutions sufficient for de novo atomic modelling.
    1. Physics of Living Systems
    2. Structural Biology and Molecular Biophysics

    High-throughput, single-particle tracking reveals nested membrane domains that dictate KRasG12D diffusion and trafficking

    Yerim Lee, Carey Phelps ... Xiaolin Nan
    The nanoscopic landscape of the membrane has a direct impact on the spatiotemporal, and likely also the signaling, dynamics of Ras proteins.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Endoplasmic reticulum stress activates human IRE1α through reversible assembly of inactive dimers into small oligomers

    Vladislav Belyy, Iratxe Zuazo-Gaztelu ... Peter Walter
    Stress sensors in the membrane of the endoplasmic reticulum respond to the accumulation of unfolded proteins by briefly forming small phosphorylation-competent oligomers and dissolving back into active dimers.
    1. Structural Biology and Molecular Biophysics

    Routine single particle CryoEM sample and grid characterization by tomography

    Alex J Noble, Venkata P Dandey ... Bridget Carragher
    Fiducial-less tomography on single particle cryoEM samples reveals that most particles are adsorbed to the air-water interface and allows for researchers to diagnose and solve sample, grid, ice thickness, collection, and processing issues.

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