The resolution of electron cryo-microscopy can be improved by using optimal exposure values to filter video frames.

The Volta phase plate enables in-focus single particle analysis at near atomic resolutions, which could expand the capabilities of cryo-EM, especially for small, flexible or heterogeneous macromolecular samples.

A freely available software environment on Amazon's cloud computing network makes processing of cryo-EM data accessible and affordable.

Multi-body refinement in RELION allows high-resolution reconstruction and visualisation of molecular motions in cryo-EM single-particle data sets with continuous forms of structural heterogeneity.

RELION-3 calculates single-particle reconstructions to higher resolution and with less user-interactions than previously available versions.

Building on previous work in cryo-electron microscopy (Entchev et al, 2015), it shown that a combination of the Volta phase plate and a small amount of defocus can simplify the experimental set-up, increase the data acquisition rate and improve resolution.

The structure of KvAP addresses a mechanism of voltage-dependent gating that has been debated for 16 years.

New hybrid structure determination methods leveraging the inherent biophysical properties of a macromolecule through molecular dynamics simulations provide accurate and cost-efficient ways of achieving atomic structures from high resolution cryo-electron density maps.

The structures of Slo1 in complex with b4 imply that the auxiliary beta subunits modulate the channel's gating properties through stabilizing ‘pre-existing’ conformations rather than creating new ones.

Hydrophilized graphene prevents protein denaturation at the air-water interface.