998 results found
    1. Developmental Biology
    2. Plant Biology

    Tracking transcription factor mobility and interaction in Arabidopsis roots with fluorescence correlation spectroscopy

    Natalie M Clark, Elizabeth Hinde ... Rosangela Sozzani
    The mechanistic basis of molecular movement and interactions within plant root cells can be quantified using scanning fluorescence correlation spectroscopy.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    The inner mechanics of rhodopsin guanylyl cyclase during cGMP-formation revealed by real-time FTIR spectroscopy

    Paul Fischer, Shatanik Mukherjee ... Peter Hegemann
    UV-Vis- and IR-spectroscopy provides insights into the light-induced enzyme activity of a rhodopsin guanylyl cyclase by tracking the substrate turnover with atomic resolution in real-time and correlating it with the structural changes of the protein.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Resolving dual binding conformations of cellulosome cohesin-dockerin complexes using single-molecule force spectroscopy

    Markus A Jobst, Lukas F Milles ... Michael A Nash
    A single-molecule approach is able to discriminate the redundant binding conformations of cellulosomal dockerin based on differences in their mechanical properties.
    1. Physics of Living Systems
    2. Structural Biology and Molecular Biophysics

    Multicolor fluorescence fluctuation spectroscopy in living cells via spectral detection

    Valentin Dunsing, Annett Petrich, Salvatore Chiantia
    Fluorescence fluctuation spectroscopy is implemented for detection of up to four molecular species, allowing users to quantify molecular interactions and stoichiometry of multicomponent complexes in live cells, in a wide range of biological processes, from membrane signaling to viral assembly.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Single-molecule force spectroscopy of protein-membrane interactions

    Lu Ma, Yiying Cai ... Yongli Zhang
    A high-resolution method to quantify interactions between lipid bilayers and single proteins under controlled load is presented and applied to key proteins involved in membrane fusion and formation and maintenance of membrane contact sites.
    1. Structural Biology and Molecular Biophysics

    Integrative dynamic structural biology unveils conformers essential for the oligomerization of a large GTPase

    Thomas-O Peulen, Carola S Hengstenberg ... Christian Herrmann
    Multimodal spectroscopy (smFRET, EPR, SAXS, and SANS) and integrative structural modeling reveal large-scale domain rearrangements in human guanylate binding protein 1 (hGBP1) that are driving forces for the formation of oligomers that enable its biological function in innate immune defense.
    1. Neuroscience

    Excitation and inhibition in anterior cingulate predict use of past experiences

    Jacqueline Scholl, Nils Kolling ... Matthew FS Rushworth
    Neurotransmitter balance in a region in frontal cortex controls how much people use information they have learnt to guide their behaviour.
    1. Physics of Living Systems

    Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy

    Antonia Franziska Eckert, Peng Gao ... Gerd Ulrich Nienhaus
    A robust fluorescence microscopy-based data acquisition and analysis framework affords the precise measurement of cell surface receptor affinities toward their cognate ligands and their densities in live cells/tissues.
    1. Neuroscience

    The trajectory of cortical GABA across the lifespan, an individual participant data meta-analysis of edited MRS studies

    Eric C Porges, Greg Jensen ... Nicolaas AJ Puts
    Individual participant data meta-analysis reveals the estimated lifespan trajectory of cortical GABA, characterized by an early rapid increase, followed by a period of stability during early adulthood, and a gradual decrease.
    1. Physics of Living Systems

    Increasing evidence of mechanical force as a functional regulator in smooth muscle myosin light chain kinase

    Fabian Baumann, Magnus Sebastian Bauer ... Hermann Eduard Gaub
    Atomic force microscopy based single-molecule force spectroscopy of smooth muscle myosin light chain kinase strongly indicates the existence of a mechanically triggerable activation pathway analogous to its well-established biochemical regulation pathway via calcium-loaded calmodulin.

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