Human cell lines replicate and proliferate without ORC1 or ORC2, two subunits of the replication initiator protein complex ORC, which has till now been considered essential for DNA replication.
Timothy J Parnell, Alisha Schlichter ... Bradley R Cairns
A combination of genetics, expression, genomic localization, and genomic nucleosome profiling demonstrates antagonism between two chromatin remodelers that reveal different mechanisms at different promoter architectures.
Shalini Gupta, Larry J Friedman ... Stephen P Bell
A single origin–recognition complex (ORC) directs loading of a pair of head-to-head Mcm2-7 replicative DNA helicases by forming a protein tether to the first helicase, releasing from its initial DNA-binding site, and rebinding the origin DNA in the opposite orientation.
The Smc–ScpAB complex-a prokaryotic ancestor of cohesin, condensin and Smc5/6-loads onto the bacterial chromosome by employing ATP hydrolysis to capture DNA fibers within its tripartite ring.
Kwun Nok Mimi Man, Peter Bartels ... Johannes W Hell
The α1-adrenergic receptor augments the activity of the L-type Ca2+ channel CaV1.2 through PKC and the tyrosine kinases Pyk2 and Src and thereby synaptic plasticity.
Christine E Cucinotta, Rachel H Dell ... Toshio Tsukiyama
Multiple mechanisms, by which a highly conserved chromatin-remodeling factor RSC facilitates initiation and maintenance of large-scale, rapid gene expression upon exit from quiescent state, have been discovered.
Nina Kirstein, Alexander Buschle ... Aloys Schepers
Replication origins are established throughout the genome with the exception of transcribed genes, and the local chromatin composition likely modulates the density of ORC and MCM as well as origin activation.
The structure of the yeast RSC complex, a member of the SWI/SNF chromatin remodeling family, determined by cryo-electron microscopy, reveals a conserved structural core and the mode of nucleosome engagement.