Two-color time-lapse superresolution imaging of the synaptic nanoorganization in vivo uncovers highly dynamic spine heads and PSD95 assemblies that are less variable in size but more dynamic in their PSD95 nanoorganization after environmental enrichment.
The discovery of a fluorescent protein that can be rapidly switched between long-lived ‘on’ and ‘off’ states will lead to a new generation of super-resolution imaging experiments on living cells.
Parisa Asghari, David RL Scriven ... Edwin DW Moore
Repositioning the type II ryanodine receptors on the sarcoplasmic reticulum membrane is a potential new mechanism regulating their function, and therefore the strength of cardiac contraction.
Javier Emperador-Melero, Giovanni de Nola, Pascal S Kaeser
Leukocyte common antigen-related receptor protein tyrosine phosphatases (LAR-RPTPs) are precisely localized to synaptic appositions, but do not control the assembly and function of the presynaptic nerve terminal.
Combined light and electron microscopy reveals a new function for Arp2/3-mediated actin assembly in nuclear envelope rupture, which leads to a separation of nuclear membranes and pores from the lamina.
An inducible two-component CRISPR-based platform that rapidly repositions HSV-1 genomes to the nuclear edge unveils intranuclear space heterogeneity for the incoming viral genomes and dynamic stages of the host-virus interplay during early infection.
Valentina Štimac, Isabella Koprivec ... Iva M Tolić
STED microscopy of human mitotic spindles reveals how augmin-nucleated microtubules protect the cell from erroneous kinetochore-microtubule attachments and ensure a highly organized architecture of the spindle required for mitotic fidelity.
STIM1 regulates the contractility of blood vessels and blood pressure by organizing membrane contact sites and ion channel activity in vascular smooth muscle cells in a manner that is independent of its canonical function in store-operated Ca2+entry.