Improved two-photon fluorescence microendoscopy enables volumetric imaging of synapses and neurons in deep brain structures in vivo.

Aberration correction using 3D microprinting in ultrathin microendoscopes allows two-photon imaging of large neuronal networks with homogeneously high spatial resolution and minimal invasiveness in the deep mouse brain.

An inference paradigm for extracting neuronal correlations from two-photon imaging data, without requiring intermediate spike deconvolution, provides significant performance gains over existing methods as demonstrated by theoretical analysis, simulation studies, and real-data applications.

Establishment of two-photon imaging with a 1100-nm laser, which underfills the objective's back aperture, detects activity of multiple neurons in the prelimbic area and hippocampal CA1 region of the intact mouse brain.

An all-optical 3D two-photon imaging and photostimulation platform was demonstrated, with the capability to precisely stimulate a large group of cells in mice cortex in vivo with low laser power.

Two-photon imaging reveals super-sparse responses to natural images in primate V1, which carry sufficient information for discrimination of the input natural images with high accuracy.

The first postnatal week ends with the development of perisomatic innervation and with an abrupt switch in the representation of self-motion in the region CA1 of the mouse hippocampus.

Computational modeling and analysis of mouse neural population data finds that the excitation/inhibition imbalance theory of brain disorders is too limited to account for key changes in neural activity statistics.

The combination of a new genetically encoded voltage indicator and fast two-photon imaging methods enables detection of rapid neural electrical activity in organotypic slice cultures and in living flies.

Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.