The cancer-associated human ubiquitin ligase HUWE1 can adopt an auto-inhibited dimeric state, whose occupancy is regulated by competing intra- and intermolecular interactions of the dimerization region and by the tumor suppressor p14ARF.

The F-box and U-box E3 ubiquitin ligase decoy library is a powerful tool for the functional characterization of redundant E3 ubiquitin ligases, like MAC3A and MAC3B, novel circadian clock regulators.

Engineered E3 ubiquitin ligases are utilized to elucidate mechanisms underlying ubiquitin regulation of membrane proteins, and to achieve robust post-translational functional knockdown of ion channels.

Interaction mapping of ubiquitin ligase complexes embedded in the endoplasmic reticulum membrane has identified interactors of RNF26 that influence innate immune signalling.

DDB1, a component of the ubiquitin proteasome system, plays a role in both hematopoietic and embryonic stem cell self-renewal and differentiation.

The general principles that underlie the mechanism of action of the COP9-Signalosome, a key regulator of the largest family of ubiquitin ligase enzymes in human cells, have been identified using structural and kinetic techniques.

Human cullin-RING ligases are buffered to a much greater extent than had been previously appreciated, and the roles of ubiquitin chain extension enzymes are far more nuanced at physiological concentrations.

CRISPR/Cas9 genome-wide screens using sterol-sensitive endogenous HMG-CoA reductase (HMGCR) reporter identify the sterol-responsive RNF145 and gp78 as independently responsible for sterol-accelerated degradation of HMGCR, the rate-limiting enzyme of cholesterol biosynthesis.

Arrestin-related trafficking adaptors (ARTs) undergo a K63 linked di-ubiquitin modification to promote Rsp5 E3 ligase recruitment and enhance cargo protein ubiquitination.

Aberrant neddylation of non-cullin substrates disrupts normal cell cycle progression.