A screen of tens of thousands of vesicle traffic networks in silico shows that Golgi-like cisternal maturation is a natural outcome of vesicle traffic homeostasis.

Electron tomography and biochemical approaches demonstrate a direct role for WDR35, beyond integrity of the IFT-A holocomplex, in the formation and fusion of electron-dense-coated vesicles to the ciliary sheath and pocket for delivery of cargos necessary for axoneme elongation.

Macropinocytosis, the process of non-specific endocytosis, is a major gateway for large volumes of surrounding medium and nanoparticles to coral cells.

Inactivation of the COPI vesicle coat in yeast reveals that COPI recycles early but not late Golgi proteins.

Phosphorylation of a core trafficking component, the COPII coat subunit Sec24, regulates cross-talk between the secretory and autophagy machinery during starvation.

Using carefully controlled expression levels and a variety of optical pulse-chase assays in mammalian neurons, an updated model for the polarized transport of synaptic vesicle proteins, and the first steps of synaptic vesicle biogenesis, were uncovered.

Atomic force microscopy reveals a floppy, dimeric, coiled coil Golgin structure that captures transport vesicles via splayed, N-terminal ends at the Golgi complex.

The same membrane trafficking cargo can change pathways mediated by different members of ARF-GEF family of vesicle formation regulators.

The priming protein ubMunc13-2 and Synaptotagmin-7 cooperate with phorbolesters/diacylglycerol to stimulate vesicle priming in adrenal chromaffin cells, whereas phorbolesters/diacylglycerol interacting with Munc13-1 inhibit vesicle fusion, which identifies opposing functions of these two Munc13 proteins.