Response to Nodal morphogen gradient is determined by the kinetics of target gene induction

  1. Julien Dubrulle  Is a corresponding author
  2. Benjamin M Jordan
  3. Laila Akhmetova
  4. Jeffrey A Farrell
  5. Seok-Hyung Kim
  6. Lilianna Solnica-Krezel
  7. Alexander F Schier  Is a corresponding author
  1. Harvard University, United States
  2. University of Minnesota, United States
  3. Medical University of South Carolina, United States
  4. Washington University School of Medicine, United States
  5. Vanderbilt University Medical Center, United States
  6. Broad Institute of MIT and Harvard, United States

Decision letter

  1. Naama Barkai
    Reviewing Editor; Weizmann Institute of Science, Israel

eLife posts the editorial decision letter and author response on a selection of the published articles (subject to the approval of the authors). An edited version of the letter sent to the authors after peer review is shown, indicating the substantive concerns or comments; minor concerns are not usually shown. Reviewers have the opportunity to discuss the decision before the letter is sent (see review process). Similarly, the author response typically shows only responses to the major concerns raised by the reviewers.

Thank you for sending your work entitled “Response to Nodal morphogen gradient is determined by the kinetics of target gene induction” for consideration at eLife. Your article has been favorably evaluated by Janet Rossant (Senior editor), a Reviewing editor, and 2 reviewers.

The Reviewing editor and the reviewers discussed their comments before we reached this decision, and the Reviewing editor has assembled the following comments to help you prepare a revised submission.

There is uniform consensus that your study addressing the mechanisms transforming the graded distribution of Nodal into distinct RNA expression-pattern domains is solid and important. This topic is central in Developmental Biology and is highly relevant for a large number of other processes during later developmental stages in which Nodal and other secreted TGF-b molecules are involved. The subject and scope of the results reported in the paper thus fit very well the requirements from papers published in eLife.

Please address the following specific points (brought in order of appearance in the text; points 5 and 6 can be viewed as the major concerns).

1) While convincing, the rescue of the MZsmad mutation by GFP-Smad2 appears incomplete as compared with the wild type and to the rescue by non-GFP version. If this really reflects the functionality of the protein, the authors should mention this point in the text.

2) “Cells close to the margin tended to activate Smad2 early and reached the highest levels of activated Smad2” (mentioned in the Results). In Figure 2E, the second and fourth cells from the margin do not appear to follow this rule. If they are exceptions, they should be replaced. Alternatively, instead of following one cell per distance, presenting the average of values measured from several adjacent cells in 50 micrometer intervals would probably provide a more convincing picture of what appears to be a correct conclusion.

3) Figure 2D: the time interval between the 5 frames presented should be indicated in the figure legend. Additionally, for the panel to be informative, at least for several representative cells, the direction of the movement should be indicated.

4) “Cells located farther away from the margin tended to activate Smad2 with a delay and the levels of activated Smad2 remained low” (in the Results). In Figure 2G, it appears that at 3.5 hpf, Smad2 is activated in cells away from the margin (80 µM and further). Subsequently, this activation is gradually lost (what appears like inactivation from 4 to 5 hpf). This is also seen in some examples shown in Figure 2E as mentioned in point 3. The '3.5 hpf results' can be interpreted as either 'noise' or as a process of inactivation with time of cells away from the source. The latter explanation (activation followed by inactivation) could be discussed in the context of the ratchet model. If the authors consider the activation values at 3.5 hpf to signify noise, they should clearly state that in the text and indicate accordingly the range of relevant data Figure 2E–G (starting from 4 hpf?).

5) “Ntl was first faintly detected in a few cells on the presumptive dorsal side of the embryo at the midblastula stage. Subsequently, its expression domain intensified and progressively extended animally until the onset of gastrulation. By contrast, gsc expression initiated ∼30 min later and remained confined to a narrow domain on the dorsal side”. As shown in Figure 2H, the authors use in situ hybridization to compare the dynamics of transcription and spatial distribution of ntl and gsc transcripts. For the conclusion to be valid, the authors should show that the sensitivity of the method is identical for both genes. This could be done for example by injecting a concentration series of a gsc-ntl fused RNA, detecting it at 3 hpf (prior to endogenous gene activation) using ntl and gsc probes (used in the study) to detect the injected RNAs. Comparable sensitivity of both probes should be confirmed. Further, identical staining procedure (e.g., duration of color development) should be applied to both probes to validate the conclusion as above. While I believe the result will be the expected one (especially in light of the nanostring data) formally, based on this panel alone and the description of the procedure, the difference in timing and expression pattern could arise from relatively more efficient ntl detection. While not essential, double labeling within the same embryo would be even more convincing.

6) Concerning Figure 3, the labeling of the host embryo as MZoep + Nodal should be changed into MZoep + nodal to indicated that the source of the Nodal is RNA. In that figure, GFP-Smad2 NC values at 5.5 hpf are interpreted by the authors to signify similar activation levels in the transplanted cells. It is possible, that cells transplanted into 3.5hpf embryos vs those transplanted into 4.5hpf embryos, could have experienced different levels of Nodal activation in the host. For example, it could in principle be that the Nodal level the transplanted cells are exposed to is higher in the “3.5hpf experiment” due to the protein expression dynamics derived from the injected RNA. To rule out this possibility, the authors could determine the N/C ratio of GFP-Smad2 at 4, 4.5 and 5 hpf as well. This experiment is suggested despite the statement that the cells do not remember the signal when transplanted into animal positions (as in Figure 3–figure supplement 1) since high initial signaling could in principle cooperate with a later weaker signal.

7) Figure 3–figure supplement 1, statistical tests showing significance for panel A would make the statement more convincing.

8) The authors state: “These analyses indicate that not only the Kd but also maximal transcription rate is a key determinant of gene-specific morphogen response...” From Figure 7C and 7D, the Kd appears to have no effect on either expression rate or expression range. It is not clear therefore why the authors consider the Kd as a contributing parameter.

9) In Figure 8B, bottom right panel, are the counts significantly higher following the earlier injection at the same developmental stage as those counts following a later injection? If so, the author should discuss this point. If the data exist, it would be interesting to discuss this point in the context of the genes analyzed in Figure 8–figure supplement 1 as shown in the right panel.

https://doi.org/10.7554/eLife.05042.033

Author response

1) While convincing, the rescue of the MZsmad mutation by GFP-Smad2 appears incomplete as compared with the wild type and to the rescue by non-GFP version. If this really reflects the functionality of the protein, the authors should mention this point in the text.

In the revised manuscript we added a supplementary table that shows that the larval lethality of Zsmad2 mutants can be rescued to adulthood by the GFP-Smad2 transgene used throughout the study and clarify that gfp-smad2 mRNA can rescue MZsmad2 mutants but not as effectively as smad2 mRNA.

2)Cells close to the margin tended to activate Smad2 early and reached the highest levels of activated Smad2(mentioned in the Results). In Figure 2E, the second and fourth cells from the margin do not appear to follow this rule. If they are exceptions, they should be replaced. Alternatively, instead of following one cell per distance, presenting the average of values measured from several adjacent cells in 50 micrometer intervals would probably provide a more convincing picture of what appears to be a correct conclusion.

We added additional cell tracks to show the general trend of Smad2 activation. We added the following text to the legend of Figure 2: “The short bursts observed in some cell tracks are caused by transient nuclear accumulation of GFP-Smad2 at the onset of nuclear envelope breakdown and are observed even in the absence of Nodal signaling.”

3) Figure 2D: the time interval between the 5 frames presented should be indicated in the figure legend. Additionally, for the panel to be informative, at least for several representative cells, the direction of the movement should be indicated.

We added this information in the legend of Figure 2. All cells move towards the vegetal pole because of epiboly movements (shown in Figure 2–figure supplement 2), but maintain their position with respect to the margin, as shown in Figure 2E.

4)Cells located farther away from the margin tended to activate Smad2 with a delay and the levels of activated Smad2 remained low(in the Results). In Figure 2G, it appears that at 3.5 hpf, Smad2 is activated in cells away from the margin (80 µM and further). Subsequently, this activation is gradually lost (what appears like inactivation from 4 to 5 hpf). This is also seen in some examples shown in Figure 2E as mentioned in point 3. The '3.5 hpf results' can be interpreted as either 'noise' or as a process of inactivation with time of cells away from the source. The latter explanation (activation followed by inactivation) could be discussed in the context of the ratchet model. If the authors consider the activation values at 3.5 hpf to signify noise, they should clearly state that in the text and indicate accordingly the range of relevant data Figure 2E–G (starting from 4 hpf?).

We added the following information to the legend of Figure 2: “Basal NC ratio is higher in younger embryos (see Figure 2G 3.5 hpf). Since this phenomenon is also observed in the absence of Nodal signaling (MZoep mutants), the higher NC ratio is unlikely to reflect early Smad2 activation, but rather a higher nuclear import/export ratio of GFP-Smad2 during early development.”

5) “Ntl was first faintly detected in a few cells on the presumptive dorsal side of the embryo at the midblastula stage. Subsequently, its expression domain intensified and progressively extended animally until the onset of gastrulation. By contrast, gsc expression initiated ∼30 min later and remained confined to a narrow domain on the dorsal side. As shown in Figure 2H, the authors use in situ hybridization to compare the dynamics of transcription and spatial distribution of ntl and gsc transcripts. For the conclusion to be valid, the authors should show that the sensitivity of the method is identical for both genes. This could be done for example by injecting a concentration series of a gsc-ntl fused RNA, detecting it at 3 hpf (prior to endogenous gene activation) using ntl and gsc probes (used in the study) to detect the injected RNAs. Comparable sensitivity of both probes should be confirmed. Further, identical staining procedure (e.g., duration of color development) should be applied to both probes to validate the conclusion as above. While I believe the result will be the expected one (especially in light of the nanostring data) formally, based on this panel alone and the description of the procedure, the difference in timing and expression pattern could arise from relatively more efficient ntl detection. While not essential, double labeling within the same embryo would be even more convincing.

We performed the experiment suggested by the reviewer and present the results in Figure 2–figure supplement 3. We found that the ntl probe is slightly better than the gsc probe but to such a small extent that it is unlikely to dramatically skew the detection of endogenous gene expression. As an additional quantitative test for gsc and ntl expression, we analyzed recent single-cell transcriptome data (Satija et al. Nature Biotechnology, in press). In this paper, we and the Regev lab mapped the expression of the zebrafish transcriptome to different bins in the 50% epiboly embryo (late blastula). As shown in Figure 2–figure supplement 4, ntl is expressed in a broader domain and at higher levels than gsc. This conclusion is also supported by the nanostring data and numerous papers that analyzed gsc and ntl expression in zebrafish and Xenopus (see for example Füller et al., Dev. Biol. 2014; Eimon & Harland, Development 2002, Bell et al., Development 2003, Webster et al., BMC Dev. Biol. 2009).

6) Concerning Figure 3, the labeling of the host embryo as MZoep + Nodal should be changed into MZoep + nodal to indicated that the source of the Nodal is RNA. In that figure, GFP-Smad2 NC values at 5.5 hpf are interpreted by the authors to signify similar activation levels in the transplanted cells. It is possible, that cells transplanted into 3.5hpf embryos vs. those transplanted into 4.5hpf embryos, could have experienced different levels of Nodal activation in the host. For example, it could in principle be that the Nodal level the transplanted cells are exposed to is higher in the3.5hpf experimentdue to the protein expression dynamics derived from the injected RNA. To rule out this possibility, the authors could determine the N/C ratio of GFP-Smad2 at 4, 4.5 and 5 hpf as well. This experiment is suggested despite the statement that the cells do not remember the signal when transplanted into animal positions (as in Figure 3–figure supplement 1) since high initial signaling could in principle cooperate with a later weaker signal.

This comment implies that cells in the 3.5 hpf experiment might have been exposed to Nodal not only for a longer time but experienced a peak of pathway activation before 5.5 hpf. As proposed by the reviewer, we measured GFP-Smad2 levels in a time course experiment and found that the NC ratio of GFP-Smad2 is not higher at any time between 3.5 and 5.5 hpf as compared to 5.5 hpf. This data is now shown in Figure 3–figure supplement 1.

7) Figure 3–figure supplement 1, statistical tests showing significance for panel A would make the statement more convincing.

Added in revised manuscript.

8) The authors state:These analyses indicate that not only the Kd but also maximal transcription rate is a key determinant of gene-specific morphogen response...From Figure 7C and 7D, the Kd appears to have no effect on either expression rate or expression range. It is not clear therefore why the authors consider the Kd as a contributing parameter.

We revised the text as follows: “Although the Kd may affect target gene response, these analyses indicate that the maximal transcription rate is a key parameter in morphogen response.” We wish to be conservative in our claims and do now want to dismiss the importance of the Kd in morphogen interpretation.

9) In Figure 8B, bottom right panel, are the counts significantly higher following the earlier injection at the same developmental stage as those counts following a later injection? If so, the author should discuss this point. If the data exist, it would be interesting to discuss this point in the context of the genes analyzed in Figure 8–figure supplement 1 as shown in the right panel.

The counts for late-expressed target genes are indeed higher following early injections. We added the following text to the legend of Figure 8: “Note that counts for the expression of late target genes are higher after early injection compared to later injections. This effect might be due to the fact that after early injections phospho-Smad2 levels are high for a longer period before a gene becomes competent to respond as compared to late injections, when there is a shorter time window of high phospho-Smad2 levels. There might be a priming mechanism in which longer exposure to activated Smad2 increases gene expression when competence is reached.”

https://doi.org/10.7554/eLife.05042.034

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  1. Julien Dubrulle
  2. Benjamin M Jordan
  3. Laila Akhmetova
  4. Jeffrey A Farrell
  5. Seok-Hyung Kim
  6. Lilianna Solnica-Krezel
  7. Alexander F Schier
(2015)
Response to Nodal morphogen gradient is determined by the kinetics of target gene induction
eLife 4:e05042.
https://doi.org/10.7554/eLife.05042

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https://doi.org/10.7554/eLife.05042