Single-cell analysis of transcription kinetics across the cell cycle

  1. Samuel O Skinner
  2. Heng Xu
  3. Sonal Nagarkar-Jaiswal
  4. Pablo R Freire
  5. Thomas P Zwaka
  6. Ido Golding  Is a corresponding author
  1. Baylor College of Medicine, United States
  2. Rice University, United States
  3. University of Illinois at Urbana-Champaign, United States
  4. Icahn School of Medicine at Mount Sinai, United States

Decision letter

  1. Robert H Singer
    Reviewing Editor; Albert Einstein College of Medicine, United States

In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.

Thank you for submitting your work entitled "Single-cell analysis of transcription kinetics across the cell cycle" for consideration by eLife. Your article has been reviewed by two peer reviewers, and the evaluation has been overseen by Rob Singer (Reviewing Editor) and Aviv Regev as the Senior Editor.

The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.

As you can see below, the reviewers were enthusiastic about the manuscript and felt it was of high quality. The need for revision centers on some items where they felt the presentation required more extensive discussion, for instance in the dosage compensation discussion and the modeling approach. We look forward to the revised manuscript soon.

Reviewer #1:

In the lovely paper "Single-cell analysis of transcription kinetics across the cell cycle" by Skinner et al., the authors investigate how transcriptional parameters of Nanog and Oct4 affect the cell-to-cell variability of these genes and how these parameters change during the cell cycle. Using single-molecule FISH measurement to precisely quantify nascent and mature RNA, and by determining the transcriptional kinetic parameters the authors show that the difference in variability between the two genes can be explained by the slower ON/OFF switching by Nanog.

I think this study is very timely, as there has been increased interest these days in the connections between global regulation of transcriptional processes and transcriptional bursts-in this case, the demonstration that there is dosage compensation upon DNA replication. The authors also have wisely chosen to study Nanog and Oct4, which has been the topic of much recent debate. One of the highlights is the authors showing that the kinetics of Nanog are what leads to the oft-described variability in Nanog transcript levels. It is also methodologically rigorous, including the RNA quantification, the modelling of the kinetic parameters the analysis, as well as the extensive documentation of the methods used.

1) The more familiar usage of the term dosage compensation comes from the case of sex chromosome dosage compensation (e.g., to balance out X chromosome dosage differences between male and female mice). I think what the authors are observing is rightly called dosage compensation, but it's probably worth mentioning the more traditional context in which the term is used and explicitly pointing out the similarities and differences.

2) The paper was exceptional in its depth of methods documentation, yet regarding the cell cycle modelling and the transcriptional kinetic parameters, the paper would benefit if the authors described some of the modeling more in the main text. For example, it would be useful to better clarify the difference between the "rough" and detailed cell-cycle analysis, possibly in a sentence at the beginning of the section. Similarly, it would be helpful if a brief explanation of the ergodic rate analysis could also be found in the main text. Along these lines: Would be helpful to define the term "cell cycle age". Also, in Figure 3C, there is no indication as to what the start and end point for "Time in cell cycle" is, and thus how the 10 time windows relate to G1, S, G2 phase.

3) One of the results I found most interesting was that the reporter did not show any dosage compensation effect. I was hoping the authors could speculate on this a bit more. In the case of Padovan-Merhar et al., they show that whatever the cause is for the dosage compensation, it's occurring in cis to the DNA, like a histone modification or something that gets diluted upon replication. It's possible that the reporter gene is not fully chromatinized, which is why it doesn't show the dosage compensation effect. Anyway, I thought it was a cool result that the authors may want to highlight more.

Reviewer #2:

Cell cycle phase is one of the most important extrinsic factors determining differences within populations of actively dividing cells. In this study Golding and colleagues combine high-quality single molecule FISH of mature and nascent mRNA and computational approaches to infer cell cycle phase and study its effect on changes in promoter burst parameters. They demonstrate their approach by identifying a dosage compensation mechanism entailing a decline in the burst frequency of the genes Nanog and Oct4. The power of this work is in the rigorous and elegant theoretical formulation of the problem of inferring burst parameters in cycling cells, and the clear description of the algorithm for extracting these parameters. I believe the methodology developed here will be instrumental to many future works related to gene expression variability in the context of the cell cycle.

The paper could be improved by addressing, at least in the text, the following points:

1) The authors should elaborate on the comparison between their results and those of Raj and colleagues (Padovan-Merhar et al., 2015). Specifically in the Padovan-Merhar paper a dosage compensation very similar to the one identified here was detected (decreased "burst frequency" upon replication), however, upon growth of cellular volume (occurring predominantly at G2) there was a global increase in number of nascent mRNA per transcription site (compensatory increase in "burst size"). The present study did not identify a difference in the burst size between G1 and G2. These discrepancies between the two works could be related to the differences in the cell lines and genes studied (specifically the shorter cell cycle time of ES cells compared to fibroblasts).

2) The deterministic model of nascent and mature mRNA kinetics (section 9) and the associated Figure 3—figure supplement 1 nicely demonstrate that the mature mRNA is not at steady state. More importantly, it shows that the mature mRNA in G2 is less than twice the levels in G1(as also shown in Figure 3C). This would mean that upon division the levels of mature mRNA at the start of G1 phase of the next round would be smaller than in the current round, and that mRNA will exponentially decline to zero with additional cycles. This naturally cannot be the case and there must be some compensatory dosage compensation somewhere along the cell cycle. While identifying this additional dosage compensation mechanism is beyond the scope of the current work it is important to note this issue in the text.

3) Section 6.1 “Quantification of DNA content”: the authors should provide the cell cycle periods for the ES cells studied, inferred by their cell cycle phase inference algorithm.

4) The authors consider a change in Kon upon replication, rather than Koff. One could imagine the dosage compensation would entail higher Koff rather than lower Kon. Would there be a potential identifiability problem in discerning between models that allow changes in both Kon and Koff?

5) The model applied assumes fixed times of replication and division, how would results change if these parameters were allowed to vary (that is if they were sampled from some normal distribution)?

6) "The number of nascent mRNA at each active transcription site was quantified in the exon-channel by dividing the integrated intensity by the integrated intensity of a single-mRNA molecule (Materials and methods 5.1)". This approach may introduce some bias that depends on the probe library design. If all probes target the first part of the gene then any RNA polymerase will have a nascent mRNA attached to it that includes the full complement of probes and thus has intensity equal to a full mature mRNA. If, however, probes are equally spread along the gene, the average RNA polymerase will have an mRNA with half of the library probes yielding a 'dimmer' dot. Correction for this effect is described in Bahar Halpern et al. 2015 and is worth considering.

https://doi.org/10.7554/eLife.12175.020

Author response

Reviewer 1:

1) The more familiar usage of the term dosage compensation comes from the case of sex chromosome dosage compensation (e.g., to balance out X chromosome dosage differences between male and female mice). I think what the authors are observing is rightly called dosage compensation, but it's probably worth mentioning the more traditional context in which the term is used and explicitly pointing out the similarities and differences.

In the revised manuscript, we now refer to the traditional context of “dosage compensation” upon first using the term. We have also expanded the discussion of our findings on this matter vis-à-vis those of Padovan-Merhar et al. (2015).

2) The paper was exceptional in its depth of methods documentation, yet regarding the cell cycle modelling and the transcriptional kinetic parameters, the paper would benefit if the authors described some of the modeling more in the main text. For example, it would be useful to better clarify the difference between the "rough" and detailed cell-cycle analysis, possibly in a sentence at the beginning of the section. Similarly, it would be helpful if a brief explanation of the ergodic rate analysis could also be found in the main text. Along these lines: Would be helpful to define the term "cell cycle age". Also, in Figure 3C, there is no indication as to what the start and end point for "Time in cell cycle" is, and thus how the 10 time windows relate to G1, S, G2 phase.

We have revised the text in a number of places to address the points highlighted by the reviewer. Specifically: (i) We clarify the distinction between the “rough” cell-cycle analysis, which consists of classifying cells into G1/S/G2, and the detailed one, which calculates a specific time within the cell cycle and the Oct4/Nanog gene copy-number for each cell. (ii) We define the function of the ergodic rate analysis. (iii) We omit the ambiguous term “cell cycle age” previously used. (iv) We clarify how the 10 time windows in Figure 3C are defined (caption to Figure 3C).

3) One of the results I found most interesting was that the reporter did not show any dosage compensation effect. I was hoping the authors could speculate on this a bit more. In the case of Padovan-Merhar et al., they show that whatever the cause is for the dosage compensation, it's occurring in cis to the DNA, like a histone modification or something that gets diluted upon replication. It's possible that the reporter gene is not fully chromatinized, which is why it doesn't show the dosage compensation effect. Anyway, I thought it was a cool result that the authors may want to highlight more.

We originally thought of this result merely as a control experiment, serving to validate the observation of dosage compensation for Oct4 and Nanog. Following the reviewer’s comment, we now briefly discuss the result and speculate that the viral enhancer elements included in the CAG promoter (Niwa et al., 1991) are more resistant to the regulatory mechanisms that create the compensatory effect in endogenous genes.

Reviewer #2:

1) The authors should elaborate on the comparison between their results and those of Raj and colleagues (Padovan-Merhar et al., 2015). Specifically in the Padovan-Merhar paper a dosage compensation very similar to the one identified here was detected (decreased "burst frequency" upon replication), however, upon growth of cellular volume (occurring predominantly at G2) there was a global increase in number of nascent mRNA per transcription site (compensatory increase in "burst size"). The present study did not identify a difference in the burst size between G1 and G2. These discrepancies between the two works could be related to the differences in the cell lines and genes studied (specifically the shorter cell cycle time of ES cells compared to fibroblasts).

As the reviewer noted, our finding that the dosage compensation following Oct4 and Nanog gene replication was achieved through a decrease in the probability of each gene copy to be active (approximately equivalent to a change in burst frequency) was very similar to what was reported by Padovan-Merhar et al. (2015) (their Figure 6A). But as also noted by the reviewer, these authors also found that the cell volume (independently of the cell cycle phase) strongly affects the number of nascent mRNAs at each transcription site (approximately equivalent to a change in burst size). We were unable to test for such an effect in our system, because the degree of cell-to-cell variability in volume within each cell-cycle phase was significantly smaller compared to Padovan-Merhar et al. (2015): CV≈0.2 (our data, not shown) versus CV≈0.5 (their Figure S3B). We therefore cannot comment on the reviewer’s hypothesis, that burst-size modulation is absent in our system due to differences in cell type of gene identity. This point is now briefly discussed in paragraph eleven, “Results & Discussion”.

2) The deterministic model of nascent and mature mRNA kinetics (section 9) and the associated Figure 3—figure supplement 1 nicely demonstrate that the mature mRNA is not at steady state. More importantly, it shows that the mature mRNA in G2 is less than twice the levels in G1(as also shown in Figure 3C). This would mean that upon division the levels of mature mRNA at the start of G1 phase of the next round would be smaller than in the current round, and that mRNA will exponentially decline to zero with additional cycles. This naturally cannot be the case and there must be some compensatory dosage compensation somewhere along the cell cycle. While identifying this additional dosage compensation mechanism is beyond the scope of the current work it is important to note this issue in the text.

As the reviewer noted, the level of mature mRNA does not reach steady state during the cell cycle, because the lifetime of mature mRNA is comparable to the duration of individual cell cycle phases. However, we should clarify that the solution displayed in Figure 3—figure supplement 1 was obtained by requiring cyclostationarity: The number of mRNA at the end of the cell cycle is twice that at the beginning of the cycle. Therefore, we do not expect our model to exhibit the compensatory dynamics described by the reviewer. The cyclostationary nature of our solution is now clarified in the caption to Figure 3—figure supplement 1.

3) Section 6.1 “Quantification of DNA content”: the authors should provide the cell cycle periods for the ES cells studied, inferred by their cell cycle phase inference algorithm.

We regret this omission. These values are now provided in paragraph two, subheading “Fitting the DNA-content distribution to a cell-cycle model and determining cell-cycle phases”.

4) The authors consider a change in Kon upon replication, rather than Koff. One could imagine the dosage compensation would entail higher Koff rather than lower Kon. Would there be a potential identifiability problem in discerning between models that allow changes in both Kon and Koff?

The reviewer is correct. The observed dosage-compensation effect is also consistent with a change in kOFF. We originally chose to invoke a change in kON as this seemed to us the most parsimonious way of explaining the observed change in number of active sites (without an accompanying change in the number of nascent mRNA per site). This choice was also motivated by the findings in previous studies (Xu et al., 2015, Senecal et al., 2014), that kON is modulated to vary expression level. But as the reviewer pointed out, our data does not allow us to distinguish whether kON or kOFF (or a combination of the two) changes following gene replication. Importantly, however, we found that our estimation of the transcription parameters for Oct4 and Nanog is insensitive to the choice between kON and kOFF as the mediators of dosage compensation. Refitting our data using a model with a kOFF-only change following gene replication gives very close results to the kON-only model in terms of the estimated kinetics, besides the expected change in the values of kON and kOFF themselves (new Figure 3—figure supplement 5). We have now revised the text on in subheading “Description of the model” and the legends of Figure 3—figure supplement 5 to reflect these points.

5) The model applied assumes fixed times of replication and division, how would results change if these parameters were allowed to vary (that is if they were sampled from some normal distribution)?

The assumption of fixed times of gene replication and cell division was made for simplicity of modeling. Naturally, these parameters are likely to vary across cells in the population. We have performed some numerical interrogation of how such variability would propagate into the observed mRNA statistics, but we feel that these preliminary studies do not reach the quality required for inclusion in the manuscript. However, the reviewer’s comment brings up a larger point: The analysis we present here is unlikely to account for all the contributions to cell-cell variability in mRNA numbers. First, as the reviewer pointed out, the parameters of the cell cycle may themselves be variable, possibly contributing to mRNA heterogeneity. Second, beyond gene dosage and the cell cycle, the stochastic kinetics of multiple processes along the life history of mRNA— elongation, splicing, nuclear export, degradation and partition—likely contribute to cell- to-cell variability in the numbers of both nascent and mature mRNA. Additional work, both experimental and theoretical, is required to delineate the contribution of these processes to mRNA heterogeneity. In the revised manuscript, we have added a Discussion paragraph to highlight this point (paragraph thirteen, Results & Discussion).

6) "The number of nascent mRNA at each active transcription site was quantified in the exon-channel by dividing the integrated intensity by the integrated intensity of a single-mRNA molecule (Materials and methods 5.1)". This approach may introduce some bias that depends on the probe library design. If all probes target the first part of the gene then any RNA polymerase will have a nascent mRNA attached to it that includes the full complement of probes and thus has intensity equal to a full mature mRNA. If, however, probes are equally spread along the gene, the average RNA polymerase will have an mRNA with half of the library probes yielding a 'dimmer' dot. Correction for this effect is described in Bahar Halpern et al. 2015 and is worth considering.

The model we used for calculating the probability distribution for the amount of nascent mRNA is based on the one we introduced in an earlier publication (Xu et al., 2015). The model explicitly takes into account the positions of smFISH probes along the gene, thus addressing the point raised by the reviewer. In the case of Oct4 and Nanog, each probe set covers 4 (for Nanog) or 5 (for Oct4) exons, as well as the 3’ UTR of the gene. We considered this coverage close enough to uniform and therefore, for simplicity, we modeled probe coverage of the gene as uniform in both cases. This point is now clarified in subheading “Calculating the nascent mRNA distributions”.

https://doi.org/10.7554/eLife.12175.021

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  1. Samuel O Skinner
  2. Heng Xu
  3. Sonal Nagarkar-Jaiswal
  4. Pablo R Freire
  5. Thomas P Zwaka
  6. Ido Golding
(2016)
Single-cell analysis of transcription kinetics across the cell cycle
eLife 5:e12175.
https://doi.org/10.7554/eLife.12175

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https://doi.org/10.7554/eLife.12175