Abstract
Behavioral strategies for foraging and reproduction in the oriental fruit fly (Bactrocera dorsalis) are alternative options for resource allocation and are controlled by neuropeptides. Here we show that the behavioral switch between foraging and reproduction is associated with changes in antennal sensitivity. Starved flies became more sensitive to food odors while suppressing their response to opposite-sex pheromones. The gene encoding sulfakinin receptor 1 (SkR1) was significantly upregulated in the antennae of starved flies, so we tested the behavioral phenotypes of null mutants for the genes encoding the receptor (skr1−/−) and its ligand sulfakinin (sk−/−). In both knockout lines, the antennal responses shifted to mating mode even when flies were starved. This suggests that sulfakinin signaling via SkR1 promotes foraging while suppressing mating. Further analysis of the mutant flies revealed that sets of odorant receptor (OR) genes were differentially expressed. Functional characterization of the differentially expressed ORs suggested that sulfakinin directly suppresses the expression of ORs that respond to opposite-sex hormones while enhancing the expression of ORs that detect food volatiles. We conclude that sulfakinin signaling via SkR1, modulating OR expressions and leading to altered antenna sensitivities, is an important component in starvation-dependent behavioral change.
Introduction
Foraging and mating behaviors are alternative options for resource allocation in insects. Starved and reproductively immature individuals intensify their foraging activity, whereas satiated and reproductively mature individuals increase their mating activity to facilitate reproduction (Chen et al., 2023; Cheriyamkunnel et al., 2021; Ebrahim et al., 2023; Ren et al., 2021). Such behavioral changes involve complex neural pathways triggered by neuropeptide signaling (González Segarra et al., 2023; Lin et al., 2022; Nässel and Zandawala, 2022; Schoofs et al., 2017; Sun et al., 2023; Zhang et al., 2022). The key regulators have been identified in the fruit fly Drosophila melanogaster, including the neuropeptides sulfakinin (Wang et al., 2022; Wu et al., 2019) and short neuropeptide F (sNPF) (Ko et al., 2015; Root et al., 2011). Tyramine and diuretic hormone 31 then resolve the conflict between foraging and mating behavior in the central nervous system (Cheriyamkunnel et al., 2021; Lin et al., 2022).
In mammals, cholecystokinin (CCK) transmits satiety by activating cholecystokinin receptor 1, leading to the expression of genes encoding other anorectic and orexigenic peptides and receptors in vagal neurons (Beutler et al., 2017; Burdyga et al., 2008; Rachel et al., 2017). CCK is also involved in the central integration of sensory inputs to regulate sexual behavior (Bloch et al., 1987; Micevych et al., 2004). In insects, sulfakinin, the ortholog of CCK, and its signals via two receptors, SkR1 (CCKLR-17D3 of D. melanogaster) and SkR2 (CCKLR-17D1), regulate sub-behavioral steps during foraging, food consumption, and mating in D. melanogaster (Fedina et al., 2023; Wang et al., 2022). For example, sulfakinin suppresses the expression of the Gr64f gustatory receptor gene, which inhibits feeding as a signal for the onset of satiety (Guo et al., 2021). Sulfakinin also inhibits both male and female sexual behavior (Wu et al., 2019). However, the complexity of sulfakinin pathways is exemplified by cell-specific signaling via SkR1 for the promotion of mating receptivity in virgin females (Wang et al., 2022).
Modulation of peripheral sensory inputs, including odorant perception, is involved in the control of mating and foraging behavior in addition to the central neuronal circuits. We therefore tested this hypothesis in the oriental fruit fly, Bactrocera dorsalis, particularly whether success in foraging and/or mating depended on the sensitivity of antennae for food volatiles or opposite-sex pheromones. We demonstrate that sulfakinin signaling via SkR1 in the antennae alters the expression of sets of odorant receptor (OR) genes during starvation enhancing the foraging success rate. Our results thus provide insight into sulfakinin signal on peripheral olfactory system that is coordinated with the central neuronal circuits for modulation of behavior.
Results
Altered antennal sensitivity is associated with starvation-induced foraging and satiation-induced mating success
We developed an assay to investigate the behavioral switch between foraging and mating induced by the physiological state of B. dorsalis (Fig. 1A, B). Foraging success increased with the duration of the starvation period (Fig. 1D, and S1), whereas courtship and copulation rates decreased, also with the starvation length-dependent manner (Fig. 1G, H). Starved flies tended to move directionally toward the food source located in the center of the arena (Fig. 1C).
To determine whether the starvation-dependent rates of foraging and mating were associated with changes in olfactory sensitivities, we surgically removed the antennae. This severely impaired mating (Fig. S2). We therefore tested antennal sensitivities toward artificial food and opposite-sex body extracts using an electroantennogram (EAG). Flies of both sexes starved for 12 h showed a greater EAG response to food odor (Fig. 1E, F) but a weaker response to opposite-sex body extracts (Fig. 1I, J). Furthermore, starved flies also showed greater EAG responses to food volatile components and weaker responses to sex pheromone components (Fig. S3). This suggested antenna-mediated olfaction is associated with and required for successful foraging and mating.
Starvation-induced SkR1 mRNA in the antennae is required for the switch from mating to foraging behavior
To identify signaling molecules responsible for the olfactory-dependent behavioral switch, we compared the expression profiles of neuropeptide signaling components in the antennae of fed and starved flies. We found that the components of three neuropeptide signaling systems were significantly upregulated in the antennae of starved flies, namely short neuropeptide F receptor (sNPFR), SIFamideR1, and SkR1, the latter showing the strongest induction (Fig. 2A).
We introduced a 7-bp deletion into the sulfakinin gene (Sk) using the CRISPR/Cas9 system, causing a frameshift that abolished the sequence region encoding two predicted mature peptides: Sk-1 and Sk-2 (Fig. 2B, S4A, B). We confirmed the absence of an anti-sulfakinin immunoreactive signal in the central nervous system of the sk−/− flies (Fig. S4C). Only SkR1 was upregulated in the starved flies among two predicted Sk receptor genes SkR1 and SkR2 (Fig. S7). We therefore generated a SkR1 null mutant (skr1−/−) by introducing a 187-bp deletion using CRISPR/Cas9, resulting in a frameshift that resulted in a premature stop codon (Fig. 2B, and S5A−C). We confirmed the absence of the anti-SkR1 immunoreactive signal in the antennae of the skr1−/− flies (Fig. S5D).
Starved sk−/− and skr1−/− flies showed the same locomotor activity as wild-type (WT) controls when we measured their velocity (Fig. 2C). However, both null mutants consumed more food than WT flies (Fig. 2D), consistent with previous results in D. melanogaster (Guo et al., 2021). Interestingly, the amount of excess food consumed by the mutants was dependent on the duration of the starvation period in both sexes. Another major difference between the null mutants and WT controls was that the mutant flies were less successful at foraging even after starvation, similar to the characteristics of satiated WT flies (Fig. 3A, B). However, foraging was not completely abolished. In addition, starved mutant females (Fig. 3C) and males (Fig. 3D) showed high mating success rates, as same rates as fed WT flies.
We next tested antennal sensitivity to food odors and body extracts by EAG in each genotype using both fed and starved flies in each case. The starved sk−/− and skr1−/− flies showed significantly weaker responses to food odors compared to the starved WT flies, although the response of sk−/− flies was slightly stronger than that of fed WT flies (Fig. 3E, F and S8), which was similar to the behavioral response for a successful foraging rate (Fig. 3A, B). The antennae of starved sk−/− and skr1−/− flies showed the same EAG response to opposite-sex body extracts as fed WT flies (Fig. 3G, H and S8), which was significantly higher than that of starved WT flies.
Sets of ORs are differentially expressed when comparing satiated/starved WT and sk−/− flies
To gain insight into the molecular basis of olfactory reprogramming by the Sk-SkR1 system, we extracted RNA from the head epidermal tissues (including antennae) of fed and starved flies for sequencing (RNA-Seq). We compared starved WT and starved sk−/−flies with satiated WT flies, focusing on the differentially expressed OR genes (Fig. 4A). We identified three OR genes (OR7a.4, OR7a.8 and OR10a) that were upregulated in the starved WT flies but unchanged in starved sk−/−flies compared to satiated WT controls, and two (OR49a and OR63a) that were downregulated in starved WT flies but unchanged in starved sk−/− flies (Fig. 4B). OR67d.3 and OR74a mRNA levels were similar in the satiated and starved WT flies. The differentially expressed genes identified by RNA-Seq were further validated by real-time reverse-transcription PCR (qRT-PCR).
Sulfakinin regulates the expression of starvation-induced ORs that detect food volatiles and starvation-suppressed ORs that detect pheromones
The OR genes identified in the RNA-Seq experiments were expressed in HEK 293 cells followed by the evaluation of functional responses to odorants using calcium mobilization assays. Tests with single high doses of volatiles representing food or sex pheromones allowed the functional categorization of ORs based on odorant specificities (Fig. 4C). OR10a was activated by ethyl benzoate (EBE), OR7a.8 was activated by ethyl butyrate (EBU) or diethyl maleate (DM), and OR7a.4 was activated by methyl eugenol (ME). The responses to these food odor volatiles were all dose-dependent, with OR10a showing the strongest response to EBE (Fig. 4E). In contrast, OR63a and OR49a showed dose-dependent responses to sex pheromone components but not to food odor volatiles (Fig. 4C, D). Therefore, the upregulated OR genes in starved WT flies, OR7a.4, OR7a.8 and OR10a, were activated by the pheromonal components, while down reulated genes, OR49a and OR63a, were activated by food volatiles. Finally, we determined whether SkR1 was expressed in OR neurons by testing the antennae for immunoreactivity to SkR1 and odorant receptor co-receptor (Orco) antisera. A subset of clustered OR neurons showed immunoreactivity to SkR1 (Fig. 4F), indicating that sulfakinin acts directly on SkR1-positive OR neurons.
Discussion
Dynamic changes in the olfactory sensitivity of insect antennae are associated with behavioral transitions in insects. Sensory inputs via olfactory perception often trigger certain behaviors, including foraging and mating (Anton and Rössler, 2021; Gadenne et al., 2016). Fine-tuning of the antennal OR repertoire would be an efficient way to co-opt behavioral outputs (Anton and Rössler, 2021; Xu et al., 2024) as we have shown here for the oriental fruit fly B. dorsalis. Changes in odorant sensitivity during transitions between foraging and mating have been reported in previous studies. For example, the antennae of female D. suzukii become significantly more sensitive to the host fruit odor isoamyl acetate after mating (Crava et al., 2019). Similarly, starved Aedes aegypti mosquitoes downregulate the sensitivity of lactic acid receptor neurons in grooved peg sensilla, which are used during host search (Davis, 1984; Oliveira et al., 2017), whereas satiated mosquitoes increase the sensitivity of OR neurons in trichoid sensilla to compounds representing the oviposition site (Siju et al., 2010). In this study, we determined the mechanisms underlying the changes in antennal sensitivity when switching between foraging and mating behavior. Sk-SkR1 signaling was required to increase the success of foraging and to suppress mating during a period of starvation. The activation of SkR1 on the antennal OR neurons upregulates the expression of ORs that detect food volatiles while suppressing those that detect pheromones.
The coordinated actions of sulfakinin on multiple downstreams, the antennae and other parts of the peripheral and central nervous system in B. dorsalis, are not yet fully understood. Sulfakinin is generally known as a satiety signal that reduces food intake and suppresses the digestive system in D. melanogaster and a number of different invertebrate species (Nässel and Wu, 2022), whereas we found that the Sk-SkR1 signal is required to promote foraging by making the antennae more sensitive to food odorants during starvation. In D. melanogaster, sulfakinin inhibits the sugar receptor GR64 in the proboscis and proleg tarsi, thus reducing food ingestion (Guo et al., 2021). Our data also support the role of sulfakinin as a satiety signal because feeding rate was increased in sk−/− and skr1−/− null mutants (Fig. 2D). Sulfakinin was shown to inhibit both male and female sexual behavior in D. melanogaster (Wu et al., 2019), in agreement with our results. The underlying mechanism is the secretion of the mature peptide Dsk-2 (but not Dsk-1) by Dsk-MP1 and Dsk-MP3 neurons, which then triggers downstream neurons expressing SkR1 to suppress male mating behavior (11). In contrast, sulfakinin was shown to promote mating receptivity in virgin females (Wang et al., 2022). The activity of sulfakinin peptides Sk1 and Sk2, through the receptors SkR1 and SkR2, is therefore likely to be complex, with spatially and temporally dynamic effects in multiple downstream systems.
Although successful foraging and EAG responses to food odors were strongly reduced in both null mutants, there was still a greater response than observed in fed WT controls (Fig. 3A, B, E, F). This incomplete suppression suggests that other mechanisms, in addition to the main Sk-SkR1 signal, are relevant to the response. Indeed, sNPF and tachykinin signaling have both been described in the modulation of olfactory sensitivity in D. melanogaster, with sNPF enhancing sensitivity to food odors and tachykinin inhibiting sensitivity to aversive odors (Ignell et al., 2009; Ko et al., 2015; Root et al., 2011). Furthermore, neuropeptide F (NPF) and SIFamide also regulate foraging behavior at the level of the olfactory circuit (Jennifer and Yi, 2013; Martelli et al., 2017; Wang et al., 2013). Notably, NPF and tachykinin also regulate mating behavior at the peripheral level. Tachykinin inhibits courtship behavior by mediating the perception of an anti-aphrodisiac pheromone in male D. melanogaster. (Shankar et al., 2015). NPF neurons are also required to detect female sex pheromones (Gendron et al., 2014; Kuo et al., 2012). Similarly, SIFamide also regulates mating behavior, although it is not yet clear whether it acts via the olfactory circuit (Sellami and Veenstra, 2015; Terhzaz et al., 2007). We have not yet investigated the roles of other neuropeptides in B. dorsalis foraging and mating behavior, but we found that starvation-induced the neuropeptide receptor genes SkR1, sNPFR, and SIFamideR1 (P < 0.05). In addition, tachykinin, SIFamide, and ion transport peptide (ITP)-like peptide were also induced by starvation, although the increase was not statistically significant. Therefore, other neuropeptides in addition to sulfakinin are likely to be involved in the switch between different sets of ORs in the antenna as a means to control behavior.
The evolutionary origin of CCK/sulfakinin signaling can be traced back to the common ancestor of the Bilateria (Mirabeau and Joly, 2013; Nässel and Wu, 2022; Staljanssens et al., 2011; Tinoco et al., 2021; Yu and Smagghe, 2014). Most coelomate and pseudocoelomate phyla produce CCK/sulfakinin peptides and orthologs of the corresponding receptors. Sulfakinin is involved in foraging, satiety, locomotion, development, aggression and reproductive behavior in insects (Fedina et al., 2023; Nässel and Wu, 2022; Nassel and Zandawala, 2019) although some insect lineages have lost both the peptide and its receptors (Consortium, 2010; Hauser et al., 2010). CCK is also involved in satiety, memory, anxiety, and depression in mammals (Jens et al., 2007; Lau et al., 2023; Rana et al., 2022; Shen et al., 2019). The many functions of CCK/sulfakinin in diverse taxonomic groups frequently include signaling between the digestive system (endoderm) and the neuronal system (ectoderm) (Nässel and Wu, 2022). This suggests an ancestral function associated with coelomic development, and that additional complexity has evolved in each lineage.
In summary, we found that the neuropeptide sulfakinin mediates the transition between foraging and mating behavior by activating the receptor SkR1 and reprogramming the peripheral OR repertoire. We propose a working model to characterize this mechanism, in which Sk-SkR1 regulates the expression of different sets of ORs, leading to the behavioral switch (Fig. 5). The modulation of peripheral sensory systems by sulfakinin, coordinating with the central neural circuit also controlled by sulfakinin, is the major trigger for a successful behavioral switch. We have thus provided new insight into the control of behavior by sulfakinin in B. dorsalis, which is one of the most widely distributed, fecund, and invasive insect pests to threaten global agriculture (Wan and Yang, 2016; Zhao et al., 2024).
Materials and methods
Insects
The wild-type (WT) oriental fruit flies were originally collected from Hainan province, China in 2008 and were reared in the laboratory (27.5 ± 0.5 °C, 75 ± 5% relative humidity, 14-h photoperiod). Larvae and adults were fed with artificial food as previously described (Shen et al., 2013). Artificial food for adults, consisting of yeast powder, honey, sugar, ascorbic acid and water, was also used as a food source for foraging behavior assays.
Behavioral assays
Virgin females and males, 3-day old, were placed each in groups of 25 flies and reared at 27.5 °C and 60% humidity, and 9-day old flies were subjected to behavioral assays. Food consumption rates of individual flies were measured by the amount Bromophenol Blue dye (BPB) in the fly after the food including 0.1% (BPB) was provided for 1 h for ad libitum feeding, which is long enough time to allow satiation to occur. BPB was measured from decapitated and homogenized bodies in 1 ml PBS buffer with 1% Triton X-100. Absorbance of the supernatant after centrifugation (13000 rpm) for 3 min was measured at 614 nm on a xMarkerTM Microplate Spectrophotometer (Bio-Rad).
Foraging behavior assay was performed according to previously published methods with modifications (Li et al., 2022). In brief, flies were starved for a certain duration in a clean mesh cage (diameter: 8 cm; height: 14cm). Behavior assay was performed after single fly was transferred individually to a clean chamber (diameter: 10 cm; height: 1cm) and acclimatized for 20 min. Then, 10 μL of artificial food was placed in the center of the chamber. Foraging success rate, the time to reach the food, was recorded by a video camera for 15 min. The experiments consisted of the assays of with 3 biological replications for each experimental condition. The behavior was analyzed by EasyFlyTracker (Qu et al., 2022) with the default parameters.
Mating behavior assay was conducted in the courtship chambers (diameter: 3.5 cm; height: 1.5cm per layer). The virgin females and males were cold anesthetized at ‒20 °C for 1 min and introduced into the courtship chamber separated by a removable transparent film. After 1 h acclimation, the film was removed to allow the paired flies to get in contact, and mating behavior was recorded by a video camera for 15 min. We measured the level of courtship in mutant males and receptivity in mutant females respectively. For courtship assay, a mutant male (sk-/- or skr-/-) and a WT virgin female were introduced into the chambers. Courtship rate is the percentage of the mutant males performs attempted copulation, which was used to measure mutant courtship level. For female receptivity assay, a mutant female (sk-/-or skr-/-) and a WT virgin male were introduced into the chambers. Copulation rate is the percentage of the mutant females had engaged in copulation, which is used to measure the mutant female receptivity. The number of flies that had performed attempted copulation or engaged in copulation by the end of each 1 min interval were summed within 15 min and plotted as a percentage of total flies for each time point.
Electroantennograms
Electroantennograms were used to record the stimulus-dependent changes in the electrical potential summed over the whole antenna. To test volatiles were food odor or body extract of each sex on the flies conditioned for satiated and starved. Different genotypes of the flies were wild-type, Sk mutant, and SkR1 mutants. Female body extracts were tested by male antennae and male body extracts by female antennae (Table S2). Body extracts were prepared by washing 15 9-day old virgin flies of each sex in 1 ml of hexane for 4 hours (Chen et al., 2023; Dweck et al., 2015; Ebrahim et al., 2023) at room temperature with gentle shaking.
For electroantennograms, similar to previous study (Li et al., 2022), the head (including the antennae) of fly was excised and a glass capillary filled with 0.9% NaCl was inserted into the cut end as the reference electrode. The tip of an antenna was placed into another glass capillary filled with 0.9% NaCl as the recording electrode. The reference electrode was connected to a grounding electrode, while the recording electrode was connected to a 10× high-impedance DC amplifier (Syntech; The Netherlands). The signal was then sent to an analog/digital converter (IDAC-4, USB, Syntech) and transferred to a computer. Finally, the data were analyzed and saved by using GC-EAD software (Syntech). A filter paper strip (3 × 0.75 cm), loaded with 10 μL of the odor solution or body extract, was placed into a pipette and delivered a stimulus into a constant, humidified airstream flowing at 100 ml/min by a stimulus controller (Syntech, Hilversum, Netherlands). Two stimuli were made for 0.5s duration with a 30s interval. Average EAG response, as shown by mV changes, was used for the calculation.
Quantitative real-time reverse transcription PCR
Antennal tissues from flies of different genotypes with different treatments were dissected and immediately stored at ‒80 °C. Total RNA was extracted with Trizol Reagent (Invitrogen) following the manufacturer’s protocol. First-strand cDNA was synthesized using the PrimeScript 1st Strand cDNA Synthesis Kit (Takara, Dalian, China) and specific primers designed using the online software, Primer 3 (http://primer3.ut.ee/, Table S1). Quantitative real-time reverse transcription PCR (qRT-PCR) was performed using a CFX Connect Real-Time System (Bio-Rad, Hercules, CA) with NovoStart SYBR PCR SuperMix kit (Novoprotein, Shanghai, China). Two reference genes (Shen et al., 2010), α-tubulin (GenBank: GU269902) and RPS3 (GenBank: XM_011212815), were used selected to normalize the expression levels for the calculation in qBASE (Hellemans et al., 2007). Each experiment comprised 3 independent biological replicates and two technical replicates.
CRISPR/Cas9-mediated mutants
In vitro synthesis of single guide RNA was performed as previously described (Li et al., 2022). In brief, the suitable target sites of Sk and SkR1 genes were searched by CasOT (Xiao et al., 2014), and the corresponding single guide RNAs (sgRNAs) were synthesized in vitro and purified using the GeneArt Precision gRNA Synthesis Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). We designed and synthesized one sgRNA for the Sk gene (Fig. S4) and two single guide RNAs for the SkR1 gene (Fig. S5).
The sgRNA and cas9 protein (Invitrogen) were gently pipetted and mixed in nuclease-free water to form a mixture of 400 ng/μL sgRNA and 300 ng/μL Cas9 protein. Eggs collected within 20 min of oviposition were injected within 2 h with the mixture of sgRNA and Cas9 protein using an IM300 Electric Microinjector (Narishige, Tokyo Japan). The injected eggs were incubated at 27.5 ± 0.5 °C and 75 ± 5% relative humidity for hatching.
Mutant screening and off-target analysis
Heterozygous G1 offspring were obtained using 9-day-old virgin adults (G0) crossed to wild-type opposite sex. Suitable mutant G1 (sk+/- and skr1+/-) were backcrossed to WT flies for at least eight generations, then homozygous mutant flies (sk-/- and skr1-/-) were obtained by self-crossing. Genomic DNA was extracted from individual flies (G0) or a single hind leg (G1 and later generations) by incubating tissue samples in 30 μL InstaGene Matrix (Bio-Rad, Hercules, CA, USA) at 56 °C for 30 min and inactivating the enzyme at 100 °C for 10 min. Fragments of 300–500 bp spanning the sgRNA target sites were amplified using the primers (Table S1). The fragments were genotyped using the QIAxcel Advanced System (Qiagen, Hilden Germany), and potential edited products were directly sequenced or cloned into vector pGEM-T Easy (Promega, Wisconsin, Madison, USA) for Sanger sequencing to identify the mutation.
To assess off-target effects, CasOT was used to search potential off-target sites for the Sk and SkR1 genes. All loci with one potential mismatch allowed in the seed region and up to three potential mismatches in the non-seed region were searched on the genome sequence (https://db.cngb.org/search/project/CNP0003192/). Two potential off-target sites were found for Sk gene and three for SkR1 gene. PCR amplification and sequencing of fragments including potential off-target sites were performed to check for mutations. In examination of the potential off-target sites with > 4 bp mismatches to the sgRNA sequences, no mutations were found in the off-sites (Fig. S6).
Immunohistochemistry
Rabbit anti-BdSkR1 antibody was generated by immunization with the peptide N’-RQGLRKCRDQWP-C’. Mouse anti-BdOrco antibody was raised by immunization with two peptides, N’-GTNPNGLTRKQE-C’ and N’-HWYDGSEEAKTF-C’. Peptide were synthesized and conjugated with keyhole limpet hemocyanin through additional cysteine residues at their N termini by Zoonbio Biotechnology (Nanjing, China). Rabbit anti-BdSk antibody was a gift from Dr. Chuan Zhou (Wu et al., 2020).
Immunohistochemistry was performed as previously described (Li et al., 2022). In brief, whole brains of 9-day-old flies were dissected in phosphate-buffered saline (PBS), fixed overnight in 4% paraformaldehyde (PFA) in PBS at 4 °C, and washed (3 × 15 min) in PBST (0.5% Triton X-100 in PBS). The tissues were blocked in 5% normal goat serum for 20 min at room temperature, then incubated in primary antibody for 48 h at 4 °C. After three washes in PBST (3 × 15 min), the tissues were then incubated in secondary antibody overnight at 4 °C. After washing in PBST again, the tissues were mounted on a slide with 100% glycerol for imaging. Adult antennae were dissected and embedded in Tissue-Tek O.C.T. Compound (Sakura, Tokyo, Japan). Sections (18 μm) were prepared using a Thermo HM525 NX microtome at ‒22 °C and then fixed in 4% PFA in PBS at room temperature for 2 h. The subsequent procedures were as described above for the brain tissues. Primary antibody used: rabbit anti-Sk (1:1000), rabbit anti-SkR1 (1:500), mouse anti-Orco (1:200). Secondary antibodies used: goat anti-rabbit IgG conjugated to Alexa Fluor 488 (1:1000, Cell Signaling Technology), goat anti-mouse IgG conjugated to Alex Fluor 647 (1:1000, Cell Signaling Technology). Samples were imaged using an LSM780 confocal microscope (Carl Zeiss, Jena, Germany) and processed with Image J software (Schneider et al., 2012).
RNA-seq analysis
Total RNA was extracted from the head epidermal tissues, including the antennae, of fed WT flies, starved WT flies, and starved sk-/- flies using TRIzol reagent (Invitrogen). Three replicates were performed. Library construction and sequencing was performed by Novogene with Illumina HiSeq2000 platform (Novogene Bioinformatics Technology Co.Ltd, Beijing, China). The raw data were analyzed after filtering the low-quality sequences by fastp with the default parameters (Chen et al., 2018). Sequences were aligned to the Bactrocera dorsalis genome (https://db.cngb.org/search/project/CNP0003192/) using Hisat2 (version 2.2.1) (Kim et al., 2015; Yang et al., 2023). Samtools (version 1.7) was used to convert sam files to bam files in the process (Li et al., 2009). Finally, to calculate the count, we use featureCounts (version 2.0.1) (Liao et al., 2014). The expression level of genes from the RNA sequencing was normalized by the FPKM method. Differential expression analysis was performed using the DESeq2 R package (1.16.1) (Anders and Huber, 2010).
Functional heterologous expression of ORs and calcium imaging
Heterologous expressions of ORs were made in Human Embryonic Kidney-293 (HEK-293) cells, which were cultured in Dulbecco’s modified Eagle medium (DMEM, 11995065, Gibco, Thermo Fisher Scientific, USA) containing 10% Fatal Bovine Serum (FBS, 10091148, Gibco) and 1% penicillin-streptomycin-Amphotericin B solution (C0224-100 ml, Beyotime) with 5% CO2 at 37 °C. Cells for transfection were seeded onto a 96-well black well plate (165305, Thermo Fisher Scientific, USA). Approximately 50%–70% confluent HEK-293 cells were transfected by 2 μg pcDNA3.1 plasmid containing respective olfactory receptors with BdOrco of B. dorsalis using TransIT®-LT1 Transfection Reagent (MIR 2300, Mirusbio) for 24 h.
For calcium imaging experiment, the culture media was replaced to 100 μl of 2.5 μM Fluo-4 AM (F14201, Invitrogen) and incubated at 37 °C with 5% CO2 for 1 h. Subsequently, after another 30 min of incubation at room temperature, the culture media containing Fluo-4 was removed, and the cells were washed three times with Hanks’ balanced salt solution (HBSS; 14065056, Gibco, Thermo Fisher Scientific, USA) in order to fully remove the Fluo-4 residue. Then, 99 ul of HBSS was added to each well and plate was placed in the dark at RT to be tested. Finally, 1 μL of test odor was added to each well and fluorescence immediately was measured for at least 80 s. Finally, images were captured by a Zeiss Observer 7 inverted microscope (Carl Zeiss, Jena, Germany). Test odors were dissolved in dimethylsulfoxide (DMSO, ST038-100ml, Beyotime) and prepared five final concentrations: 10−7, 10−6, 10−5, 10−4 M and 10−3 M. the fluorescent ratio (ΔF/F) was calculated as peak intensity divided by basal intensity (intensity before ligand perfusion). For all experiment, the response to DMSO was used as negative control.
Statistical analysis
For qRT-PCR result analysis, relative expression levels were calculated using qBase (Hellemans et al., 2007) based on the expression of the reference genes. Statistical analysis is performed using GraphPad Prism and indicated in each figure legend. Data were first verified for normal distribution using the Shapiro-Wilk normality test. One-way ANOVA, followed by Tukey’s multiple comparisons (if normal distribution) and Kruskal-Wallis and post-hoc Mann-Whitney U-tests (if non-normal distribution), was used for comparisons among multiple groups. Values are reported as means ± standard errors of the mean (SEM).
Acknowledgements
This research was supported by funding from the National Natural Science Foundation of China (32072491, 31772233), National Key R&D Program of China (2022YFC2601000), 111 Project (B18044), and the China Agriculture Research System of MOF and MARA. We are grateful to Dr. Chuan Zhou and Dr. Fengming Wu (Institute of Zoology, Chinese Academy of Sciences) for the DSK antibody. We thank Dr. Tao Wang (Institute of Zoology, Chinese Academy of Sciences) for her graphical assistance for this manuscript.
References
- 1.Differential expression analysis for sequence count dataGenome Biol 11
- 2.Plasticity and modulation of olfactory circuits in insectsCell Tissue Res 383:149–164
- 3.Dynamics of Gut-Brain Communication Underlying HungerNeuron 96:461–475
- 4.Cholecystokinin stimulates and inhibits lordosis behavior in female ratsPhysiol. Behav 39:217–224
- 5.Cholecystokinin regulates expression of Y2 receptors in vagal afferent neurons serving the stomachJ. Neurosci 28:11583–11592
- 6.. fastp: an ultra-fast all-in-one FASTQ preprocessorBioinformatics 34:i884–i890
- 7.Female semiochemicals stimulate male courtship but dampen female sexual receptivityProc. Natl. Acad. Sci. U. S. A 120
- 8.A neuronal mechanism controlling the choice between feeding and sexual behaviors in DrosophilaCurr. Biol 31:4231–4245
- 9.Genome Sequence of the Pea Aphid Acyrthosiphon pisumPLOS Biol 8
- 10.Functional transcriptome analyses of Drosophila suzukii antennae reveal mating-dependent olfaction plasticity in femalesInsect Biochem. Mol. Biol 105:51–59
- 11.Regulation of sensitivity in the peripheral chemoreceptor systems for host-seeking behaviour by a haemolymph-borne factor in Aedes aegyptiJ. Insect Physiol 30:179–183
- 12.Pheromones mediating copulation and attraction in DrosophilaProc. Natl. Acad. Sci. U. S. A 112:E2829–2835
- 13.A volatile sex attractant of tsetse fliesScience 379
- 14.The neuropeptide drosulfakinin enhances choosiness and protects males from the aging effects of social perceptionProc. Natl. Acad. Sci. U. S. A 120
- 15.Plasticity in Insect Olfaction: To Smell or Not to Smell?Annu. Rev. Entomol 61:317–333
- 16.Drosophila Life Span and Physiology Are Modulated by Sexual Perception and RewardScience 343:544–548
- 17.Hunger- and thirst-sensing neurons modulate a neuroendocrine network to coordinate sugar and water ingestioneLife 12
- 18.Cholecystokinin-like peptide mediates satiety by inhibiting sugar attractionPLoS Genet 17
- 19.Genomics and Peptidomics of Neuropeptides and Protein Hormones Present in the Parasitic Wasp Nasonia vitripennisJ. Proteome Res 9:5296–5310
- 20.qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR dataGenome Biol 8
- 21.Presynaptic peptidergic modulation of olfactory receptor neurons in drosophilaProc. Natl. Acad. Sci. U. S. A 106:13070–13075
- 22.Graded encoding of food odor value in the Drosophila brainJ. Neurosci 33
- 23.The biology of cholecystokinin and gastrin peptidesCurr. Top. Med. Chem 7:1154–1165
- 24.HISAT: a fast spliced aligner with low memory requirementsNat. Methods 12:357–360
- 25.Starvation promotes concerted modulation of appetitive olfactory behavior via parallel neuromodulatory circuitsElife 4
- 26.Insulin Signaling Mediates Sexual Attractiveness in DrosophilaPLOS Genet 8
- 27.The potential role of the cholecystokinin system in declarative memoryNeurochem. Int 162
- 28.The Sequence Alignment/Map format and SAMtoolsBioinformatics 25:2078–2079
- 29.The short neuropeptide F receptor regulates olfaction-mediated foraging behavior in the oriental fruit fly Bactrocera dorsalis (Hendel)Insect Biochem. Mol. Biol 140
- 30.featureCounts: an efficient general purpose program for assigning sequence reads to genomic featuresBioinformatics 30:923–930
- 31.A nutrient-specific gut hormone arbitrates between courtship and feedingNature 602:632–638
- 32.SIFamide Translates Hunger Signals into Appetitive and Feeding Behavior in DrosophilaCell Rep 20:464–478
- 33.Distribution of cholecystokinin-immunoreactive cell bodies in the male and female rat: II. Bed nucleus of the stria terminalis and amygdalaJ. Comp. Neurol 269:381–391
- 34.Molecular evolution of peptidergic signaling systems in bilateriansProc. Natl. Acad. Sci. U. S. A 110:E2028–E2037
- 35.Cholecystokinin/sulfakinin peptide signaling: conserved roles at the intersection between feeding, mating and aggressionCell Mol. Life Sci 79
- 36.Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behaviorProg Neurobiol 179
- 37.Endocrine cybernetics: neuropeptides as molecular switches in behavioural decisionsOpen Biol 12
- 38.Feeding-induced changes in allatostatin-A and short neuropeptide F in the antennal lobes affect odor-mediated host seeking in the yellow fever mosquito, Aedes aegyptiPlos One 12
- 39.EasyFlyTracker: A simple video tracking python package for analyzing adult Drosophila locomotor and sleep activity to facilitate revealing the effect of psychiatric drugsFront. Behav. Neurosci 15
- 40.AgRP Neurons Can Increase Food Intake during Conditions of Appetite Suppression and Inhibit Anorexigenic Parabrachial NeuronsJ. Neurosci 37
- 41.Exploring the role of neuropeptides in depression and anxietyProg. Neuropsychopharmacol. Biol. Psychiatry 114
- 42.Rectal bacteria produce sex pheromones in the male oriental fruit flyCurr. Biol 31:2220–2226
- 43.Presynaptic facilitation by neuropeptide signaling mediates odor-driven food searchCell 145:133–144
- 44.NIH Image to ImageJ: 25 years of image analysisNat. Methods 9:671–675
- 45.Neuropeptides as Regulators of Behavior in InsectsAnnu. Rev. Entomol 62:35–52
- 46.SIFamide acts on fruitless neurons to modulate sexual behavior in Drosophila melanogasterPeptides 74:50–56
- 47.The neuropeptide tachykinin is essential for pheromone detection in a gustatory neural circuiteLife 4
- 48.Cannabinoid CB1 receptors in the amygdalar cholecystokinin glutamatergic afferents to nucleus accumbens modulate depressive-like behaviorNat. Med 25:337–349
- 49.In silico cloning and annotation of genes involved in the digestion, detoxification and RNA interference mechanism in the midgut of Bactrocera dorsalis [Hendel (Diptera: Tephritidae)]Insect Mol. Biol 22:354–365
- 50.Evaluation of endogenous references for gene expression profiling in different tissues of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae)BMC Mol. Biol 11
- 51.Influence of blood meal on the responsiveness of olfactory receptor neurons in antennal sensilla trichodea of the yellow fever mosquito, Aedes aegyptiJ. Insect Physiol 56:659–665
- 52.The CCK(-like) receptor in the animal kingdom: functions, evolution and structuresPeptides 32:607–619
- 53.A neural pathway underlying hunger modulation of sexual receptivity in Drosophila femalesCell Rep 42
- 54.The neuropeptide SIFamide modulates sexual behavior in DrosophilaBiochem. Biophys. Res. Commun 352:305–310
- 55.Ancient role of sulfakinin/cholecystokinin-type signalling in inhibitory regulation of feeding processes revealed in an echinodermeLife 10
- 56.Invasion and management of agricultural alien insects in ChinaAnnu. Rev. Entomol 61:77–98
- 57.Drosulfakinin signaling modulates female sexual receptivity in DrosophilaElife 11
- 58.Neuropeptide-Gated Perception of Appetitive Olfactory Inputs in Drosophila LarvaeCell Rep 3:820–830
- 59.A neuropeptide regulates fighting behavior in Drosophila melanogasterElife 9
- 60.Drosulfakinin signaling in fruitless circuitry antagonizes P1 neurons to regulate sexual arousal in DrosophilaNat. Commun 10
- 61.CasOT: a genome-wide Cas9/gRNA off-target searching toolBioinformatics 30:1180–1182
- 62.Plasticity of the olfactory behaviors in Bactrocera dorsalis under various physiological states and environmental conditionsCurr. Opin. Insect Sci 63
- 63.Chromosome-level genome assembly reveals potential epigenetic mechanisms of the thermal tolerance in the oriental fruit fly, Bactrocera dorsalisInt. J. Biol. Macromol 225:430–441
- 64.CCK(-like) and receptors: structure and phylogeny in a comparative perspectiveGen. Comp. Endocrinol 209:74–81
- 65.Nutrients and pheromones promote insulin release to inhibit courtship driveSci. Adv 8
- 66.The Global Epidemic of Bactrocera Pests: Mixed-Species Invasions and Risk AssessmentAnnu. Rev. Entomol 69:219–237
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